Detail Information for IndEnz0002016998
IED ID IndEnz0002016998
Enzyme Type ID protease016998
Protein Name ATP-dependent Clp protease ATP-binding subunit ClpE
ATPase ClpE
Heat shock protein HSP1
Gene Name clpE BSU13700
Organism Bacillus subtilis (strain 168)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis (strain 168)
Enzyme Sequence MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPKEQSAAKQSEKKGLLDELAQNITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVANTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAALERRFQPVMVQEPSIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELNDEDAAERLTAIEAEKTKALEEENYELAAKLRDEELALEKKLNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKMKELEAKLHERVIGQEAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYMEKHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSNAGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRFDSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTDEAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK
Enzyme Length 699
Uniprot Accession Number O31673
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: ATPase essential both for efficient CtsR-dependent gene derepression during heat stress and for rerepression. Together with ClpP, degrades the global regulator CtsR after heat shock. Is also involved in disaggregation of heat-denatured proteins. Has thus a role in overall protein quality control in response to heat stress. {ECO:0000269|PubMed:16788169}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding NP_BIND 119..126; /note=ATP; /evidence=ECO:0000255; NP_BIND 446..453; /note=ATP; /evidence=ECO:0000255
Features Chain (1); Domain (1); Mutagenesis (1); Nucleotide binding (2); Zinc finger (1)
Keywords ATP-binding;Chaperone;Metal-binding;Nucleotide-binding;Reference proteome;Stress response;Zinc;Zinc-finger
Interact With
Induction INDUCTION: By heat shock via the relief of repression carried out by the transcriptional regulator CtsR. {ECO:0000269|PubMed:10320580, ECO:0000269|PubMed:14679237}.
Subcellular Location
Modified Residue
Post Translational Modification PTM: ClpE is a very short-lived protein, that is, at least in the soluble cell fraction, degraded mainly by ClpCP.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 77,906
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda