IED ID | IndEnz0002017049 |
Enzyme Type ID | protease017049 |
Protein Name |
Chloroplastic ATP-dependent Clp protease proteolytic subunit 1 EC 3.4.21.92 Endopeptidase ClpP1 pClpP |
Gene Name | clpP1 ClpP AtCg00670 |
Organism | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
Enzyme Sequence | MPIGVPKVPFRSPGEGDTSWVDIYNRLYRERLFFLGQEVDTEISNQLISLMIYLSIEKDTKDLYLFINSPGGWVISGMAIYDTMQFVRPDVQTICMGLAASIASFILVGGAITKRIAFPHARVMIHQPASSFYEAQTGEFILEAEELLKLRETITRVYVQRTGKPIWVISEDMERDVFMSATEAQAYGIVDLVAVQ |
Enzyme Length | 196 |
Uniprot Accession Number | P56772 |
Absorption | |
Active Site | ACT_SITE 101; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_00444; ACT_SITE 126; /evidence=ECO:0000255|HAMAP-Rule:MF_00444 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).; EC=3.4.21.92; Evidence={ECO:0000255|HAMAP-Rule:MF_00444}; |
DNA Binding | |
EC Number | 3.4.21.92 |
Enzyme Function | FUNCTION: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins. {ECO:0000255|HAMAP-Rule:MF_00444}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (2); Chain (1) |
Keywords | Chloroplast;Direct protein sequencing;Hydrolase;Plastid;Protease;RNA editing;Reference proteome;Serine protease |
Interact With | |
Induction | INDUCTION: Repressed in darkness. Slightly induced by high light stress and during cold acclimation (at protein level). {ECO:0000269|PubMed:10427773, ECO:0000269|PubMed:11982939}. |
Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000269|PubMed:10427773, ECO:0000269|PubMed:11352464, ECO:0000269|PubMed:11982939, ECO:0000269|PubMed:14593120}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | 16705403; |
Motif | |
Gene Encoded By | Chloroplast |
Mass | 22,124 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda | 3.4.21.92; |