| IED ID | IndEnz0002017083 |
| Enzyme Type ID | protease017083 |
| Protein Name |
ATP-dependent Clp protease proteolytic subunit 1, mitochondrial EC 3.4.21.92 Endopeptidase Clp |
| Gene Name | clpp-1 ZK970.2 |
| Organism | Caenorhabditis elegans |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans |
| Enzyme Sequence | MLRRLVTSSLSASRSMSASVQSRVGIPFVIDNEGKGERTYDIYSRLLRDRIVCLMTPVDDFIASALIAQLLFLQSESGKKPIHMYINSPGGSVTAGLAIYDTIQMISAPVSTWVIGQASSMGSLLLCAGEKGMRSALPNSRIMVHQPSGGAQGTCSDIVIRAEEITRLKRRLNEIYVHHTGMSYDEIEKTLDRDRFMSAHEALKFGLVDQIETHNGSMPSD |
| Enzyme Length | 221 |
| Uniprot Accession Number | Q27539 |
| Absorption | |
| Active Site | ACT_SITE 120; /note=Nucleophile; /evidence=ECO:0000305; ACT_SITE 145; /evidence=ECO:0000305 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).; EC=3.4.21.92; |
| DNA Binding | |
| EC Number | 3.4.21.92 |
| Enzyme Function | FUNCTION: Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. Clp may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. {ECO:0000269|PubMed:17925224}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (2); Chain (1); Transit peptide (1) |
| Keywords | Alternative splicing;Hydrolase;Mitochondrion;Protease;Reference proteome;Serine protease;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000269|PubMed:17925224}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14551910; 15791247; 17486083; 17704769; 18082681; 20188671; 21085631; 21177967; 22286215; 22560298; 23800452; 25487147; 26913604; 27133166; 27457958; 27610575; |
| Motif | |
| Gene Encoded By | |
| Mass | 24,266 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.92; |