IED ID | IndEnz0002017111 |
Enzyme Type ID | protease017111 |
Protein Name |
2S albumin-like cysteine protease inhibitor AaCI-2S Fragments |
Gene Name | |
Organism | Araucaria angustifolia (Brazilian pine tree) |
Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Acrogymnospermae Pinopsida Pinidae Conifers II Araucariaceae Araucaria Araucaria angustifolia (Brazilian pine tree) |
Enzyme Sequence | ERRCDPRRLSDCEDFVRGRSKGGRGEKECRLSERCCTELQKMPRECRCEAVEGMYKEAERKERGEGEQRQRLERARALPGLCSIEPSYCEIRPS |
Enzyme Length | 94 |
Uniprot Accession Number | C0HLT8 |
Absorption | |
Active Site | |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | |
DNA Binding | |
EC Number | |
Enzyme Function | FUNCTION: Cysteine protease inhibitor that likely functions in defense against insects by inhibiting cysteine proteases in the midgut of herbivore insects such as C.maculatus (PubMed:33266031). Selectively inhibits cathepsin L, as well as papain, ficin and bromelain with lower efficiency (PubMed:33266031). Shows antitumor activity, inhibiting the growth of prostate cancer cell lines PC3 and DU145, and the gastric cancer cell line Hs746T (PubMed:33266031). No activity against cathepsin B or serine proteases (trypsin, human plasma kallikrein and elastase) (PubMed:33266031). {ECO:0000269|PubMed:33266031}. |
Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Thermostable. Active from 25 to 100 degrees Celsius. Retains 80% of its maximal activity after heating for 2 hours at 100 degrees and retains around 30% of its maximal activity after heating for 4 hours at 100 degrees. {ECO:0000269|PubMed:33266031}; |
PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is around 6. Stable from pH 2 to 10. {ECO:0000269|PubMed:33266031}; |
Pathway | |
nucleotide Binding | |
Features | Chain (1); Disulfide bond (3); Non-adjacent residues (2); Non-terminal residue (2) |
Keywords | Direct protein sequencing;Disulfide bond;Protease inhibitor |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 11,012 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |