Detail Information for IndEnz0002017140
IED ID IndEnz0002017140
Enzyme Type ID protease017140
Protein Name Chymase
EC 3.4.21.39
Alpha-chymase
Mast cell chymase 1
Mast cell protease 5
mMCP-5
Mast cell protease I
Gene Name Cma1 Mcpt5
Organism Mus musculus (Mouse)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse)
Enzyme Sequence MHLLTLHLLLLLLGSSTKAGEIIGGTECIPHSRPYMAYLEIVTSENYLSACSGFLIRRNFVLTAAHCAGRSITVLLGAHNKTSKEDTWQKLEVEKQFLHPKYDENLVVHDIMLLKLKEKAKLTLGVGTLPLSANFNFIPPGRMCRAVGWGRTNVNEPASDTLQEVKMRLQEPQACKHFTSFRHNSQLCVGNPKKMQNVYKGDSGGPLLCAGIAQGIASYVHRNAKPPAVFTRISHYRPWINKILREN
Enzyme Length 247
Uniprot Accession Number P21844
Absorption
Active Site ACT_SITE 66; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 110; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 203; /note=Charge relay system; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa.; EC=3.4.21.39;
DNA Binding
EC Number 3.4.21.39
Enzyme Function FUNCTION: Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Disulfide bond (3); Domain (1); Erroneous initiation (1); Glycosylation (1); Propeptide (1); Sequence conflict (3); Signal peptide (1)
Keywords Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:1527387}. Cytoplasmic granule {ECO:0000269|PubMed:1527387}. Note=Secretory granules.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..19; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 10737774; 12444981; 14578624; 15905575; 15919053; 16777228; 16807745; 16807746; 17142727; 18353771; 18421012; 18753310; 19470876; 19556720; 19720934; 20016109; 20091777; 20864640; 21076070; 21267068; 21677750; 22223678; 22627280; 23306080; 24523504; 24814421; 27302551; 28193842; 29529050; 7959952; 8018917; 8098710; 8996238; 9360993; 9376586; 9379034; 9541598;
Motif
Gene Encoded By
Mass 27,586
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda 3.4.21.B5;