Detail Information for IndEnz0002017147
IED ID IndEnz0002017147
Enzyme Type ID protease017147
Protein Name Chymase
EC 3.4.21.39
Alpha-chymase
Gene Name CMA1
Organism Oryctolagus cuniculus (Rabbit)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Lagomorpha Leporidae (rabbits and hares) Oryctolagus Oryctolagus cuniculus (Rabbit)
Enzyme Sequence EIIGGTESKPHSRPYMAHLKIITKQGTFAYCGGFLISREFVMTAAHCKGRHITVTLGAHDVVKKESTWQKIDVVKQFVHPNYNSYTIRHDVMLLKLKEKANLTLTVGTLPLLPQSNFIPPGRMCRAVGWGKTSVKESHSNVLQEVKLRLLDPPACQHFPDFNHNLQLCMGTVFKGDSGPLLCARVAQGIASYAHKNAMPPSVFTRISYYRPWINKVLKEN
Enzyme Length 220
Uniprot Accession Number P85202
Absorption
Active Site ACT_SITE 46; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P23946; ACT_SITE 90; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P23946; ACT_SITE 177; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P23946
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa.; EC=3.4.21.39; Evidence={ECO:0000250|UniProtKB:P23946};
DNA Binding
EC Number 3.4.21.39
Enzyme Function FUNCTION: Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. {ECO:0000250|UniProtKB:P23946}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Disulfide bond (3); Domain (1); Glycosylation (1)
Keywords Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Serine protease
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P23946}. Cytoplasmic granule {ECO:0000250|UniProtKB:P23946}. Note=Mast cell granules. {ECO:0000250|UniProtKB:P23946}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 24,710
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda