Detail Information for IndEnz0002017211
IED ID IndEnz0002017211
Enzyme Type ID protease017211
Protein Name ATP-dependent Clp protease proteolytic subunit 1
EC 3.4.21.92
Endopeptidase Clp 1
Gene Name clpP1 SCO2619 SCC80.04c
Organism Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces albidoflavus group Streptomyces coelicolor Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Enzyme Sequence MRRPGAVVRRAGGYVTNLMPSAAGEPSIGGGLGDQVYNRLLGERIIFLGQPVDDDIANKITAQLLLLASDPDKDIFLYINSPGGSITAGMAIYDTMQYIKNDVVTIAMGLAASMGQFLLSAGTPGKRFALPNAEILIHQPSAGLAGSASDIKIHAERLLHTKRRMAELTSQHTGQTIEQITRDSDRDRWFDAFEAKEYGLIDDVIATAAGMPGGGGTGA
Enzyme Length 219
Uniprot Accession Number Q9F315
Absorption
Active Site ACT_SITE 113; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_00444; ACT_SITE 138; /evidence=ECO:0000255|HAMAP-Rule:MF_00444
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).; EC=3.4.21.92; Evidence={ECO:0000255|HAMAP-Rule:MF_00444};
DNA Binding
EC Number 3.4.21.92
Enzyme Function FUNCTION: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity). Probably partially responsible for degradation of ECF sigma factor SigR prime. {ECO:0000255|HAMAP-Rule:MF_00444, ECO:0000269|PubMed:19682253}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Sequence conflict (1)
Keywords Cytoplasm;Hydrolase;Protease;Reference proteome;Serine protease
Interact With
Induction INDUCTION: Expressed from 2 promoters, 1 of which (clpP2) is positively regulated by the thio-oxidizing agent diamide, probably via SigR. Probably a clpP1-clpP2 operon. {ECO:0000269|PubMed:19682253}.
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00444}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 23,229
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda