Detail Information for IndEnz0002017242
IED ID IndEnz0002017242
Enzyme Type ID protease017242
Protein Name ATP-dependent Clp protease proteolytic subunit 1
EC 3.4.21.92
Endopeptidase Clp 1
Gene Name clpP1 clpP-1 TP_0507
Organism Treponema pallidum (strain Nichols)
Taxonomic Lineage cellular organisms Bacteria Spirochaetes Spirochaetia Spirochaetales Treponemataceae Treponema Treponema pallidum Treponema pallidum subsp. pallidum (syphilis treponeme) Treponema pallidum (strain Nichols)
Enzyme Sequence MRERMHNLVPYVIEQSGGGERSYDIFSRLLKDRIIFVDGEITDAVADLVVAQLLFLESQNPDKDISLYINSPGGAVTAGLAVYDTMQHICPEVQTICLGQASSMAAVLLAGGAPGKRFALPSSRVMIHQPWGGVQGQASDVCIQAQEILRLKTLTIAYFALHTGQSEEQVREDMERDFFLSAEQACSYGIVDTVMKRRKHAQV
Enzyme Length 203
Uniprot Accession Number O83520
Absorption
Active Site ACT_SITE 103; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_00444; ACT_SITE 128; /evidence=ECO:0000255|HAMAP-Rule:MF_00444
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).; EC=3.4.21.92; Evidence={ECO:0000255|HAMAP-Rule:MF_00444};
DNA Binding
EC Number 3.4.21.92
Enzyme Function FUNCTION: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins. {ECO:0000255|HAMAP-Rule:MF_00444}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1)
Keywords Cytoplasm;Hydrolase;Protease;Reference proteome;Serine protease
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00444}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 18509523;
Motif
Gene Encoded By
Mass 22,346
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda