| IED ID | IndEnz0002017261 |
| Enzyme Type ID | protease017261 |
| Protein Name |
ATP-dependent Clp protease proteolytic subunit 2, mitochondrial EC 3.4.21.92 ATP-dependent Clp protease proteolytic subunit 1, mitochondrial Endopeptidase ClpP2 nClpP7 |
| Gene Name | CLPP2 NCLPP7 At5g23140 MYJ24.13 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MMRGLVSGAKMLSSTPSSMATSIATGRRSYSLIPMVIEHSSRGERAYDIFSRLLKERIICINGPINDDTSHVVVAQLLYLESENPSKPIHMYLNSPGGHVTAGLAIYDTMQYIRSPISTICLGQAASMASLLLAAGAKGQRRSLPNATVMIHQPSGGYSGQAKDITIHTKQIVRVWDALNELYVKHTGQPLDVVANNMDRDHFMTPEEAKAFGIIDEVIDERPLELVKDAVGNESKDKSSS |
| Enzyme Length | 241 |
| Uniprot Accession Number | Q9FN42 |
| Absorption | |
| Active Site | ACT_SITE 127; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q27539; ACT_SITE 152; /evidence=ECO:0000250|UniProtKB:Q27539 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).; EC=3.4.21.92; |
| DNA Binding | |
| EC Number | 3.4.21.92 |
| Enzyme Function | FUNCTION: Component of the mitochondrial ATP-dependent Clp protease (PubMed:11352464). Cleaves peptides in various proteins in a process that requires ATP hydrolysis (By similarity). Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity). {ECO:0000250|UniProtKB:Q27539, ECO:0000269|PubMed:11352464}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Transit peptide (1) |
| Keywords | Direct protein sequencing;Hydrolase;Mitochondrion;Protease;Reference proteome;Serine protease;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:11352464, ECO:0000269|PubMed:14593120, ECO:0000305|PubMed:25732537}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11743115; 12185496; 14671022; 15829605; 16895613; 17181860; 17828791; 20018591; 28627464; 32571844; |
| Motif | |
| Gene Encoded By | |
| Mass | 26,283 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |