Detail Information for IndEnz0002017261
IED ID IndEnz0002017261
Enzyme Type ID protease017261
Protein Name ATP-dependent Clp protease proteolytic subunit 2, mitochondrial
EC 3.4.21.92
ATP-dependent Clp protease proteolytic subunit 1, mitochondrial
Endopeptidase ClpP2
nClpP7
Gene Name CLPP2 NCLPP7 At5g23140 MYJ24.13
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MMRGLVSGAKMLSSTPSSMATSIATGRRSYSLIPMVIEHSSRGERAYDIFSRLLKERIICINGPINDDTSHVVVAQLLYLESENPSKPIHMYLNSPGGHVTAGLAIYDTMQYIRSPISTICLGQAASMASLLLAAGAKGQRRSLPNATVMIHQPSGGYSGQAKDITIHTKQIVRVWDALNELYVKHTGQPLDVVANNMDRDHFMTPEEAKAFGIIDEVIDERPLELVKDAVGNESKDKSSS
Enzyme Length 241
Uniprot Accession Number Q9FN42
Absorption
Active Site ACT_SITE 127; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q27539; ACT_SITE 152; /evidence=ECO:0000250|UniProtKB:Q27539
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).; EC=3.4.21.92;
DNA Binding
EC Number 3.4.21.92
Enzyme Function FUNCTION: Component of the mitochondrial ATP-dependent Clp protease (PubMed:11352464). Cleaves peptides in various proteins in a process that requires ATP hydrolysis (By similarity). Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity). {ECO:0000250|UniProtKB:Q27539, ECO:0000269|PubMed:11352464}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Transit peptide (1)
Keywords Direct protein sequencing;Hydrolase;Mitochondrion;Protease;Reference proteome;Serine protease;Transit peptide
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:11352464, ECO:0000269|PubMed:14593120, ECO:0000305|PubMed:25732537}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 11743115; 12185496; 14671022; 15829605; 16895613; 17181860; 17828791; 20018591; 28627464; 32571844;
Motif
Gene Encoded By
Mass 26,283
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda