| IED ID | IndEnz0002017283 |
| Enzyme Type ID | protease017283 |
| Protein Name |
Complement C2 EC 3.4.21.43 C3/C5 convertase Cleaved into: Complement C2b fragment; Complement C2a fragment |
| Gene Name | C2 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MGPLMVLFCLLFLYPGLADSAPSCPQNVNISGGTFTLSHGWAPGSLLTYSCPQGLYPSPASRLCKSSGQWQTPGATRSLSKAVCKPVRCPAPVSFENGIYTPRLGSYPVGGNVSFECEDGFILRGSPVRQCRPNGMWDGETAVCDNGAGHCPNPGISLGAVRTGFRFGHGDKVRYRCSSNLVLTGSSERECQGNGVWSGTEPICRQPYSYDFPEDVAPALGTSFSHMLGATNPTQKTKESLGRKIQIQRSGHLNLYLLLDCSQSVSENDFLIFKESASLMVDRIFSFEINVSVAIITFASEPKVLMSVLNDNSRDMTEVISSLENANYKDHENGTGTNTYAALNSVYLMMNNQMRLLGMETMAWQEIRHAIILLTDGKSNMGGSPKTAVDHIREILNINQKRNDYLDIYAIGVGKLDVDWRELNELGSKKDGERHAFILQDTKALHQVFEHMLDVSKLTDTICGVGNMSANASDQERTPWHVTIKPKSQETCRGALISDQWVLTAAHCFRDGNDHSLWRVNVGDPKSQWGKEFLIEKAVISPGFDVFAKKNQGILEFYGDDIALLKLAQKVKMSTHARPICLPCTMEANLALRRPQGSTCRDHENELLNKQSVPAHFVALNGSKLNINLKMGVEWTSCAEVVSQEKTMFPNLTDVREVVTDQFLCSGTQEDESPCKGESGGAVFLERRFRFFQVGLVSWGLYNPCLGSADKNSRKRAPRSKVPPPRDFHINLFRMQPWLRQHLGDVLNFLPL |
| Enzyme Length | 752 |
| Uniprot Accession Number | P06681 |
| Absorption | |
| Active Site | ACT_SITE 507; /note=Charge relay system; /evidence=ECO:0000269|PubMed:17027507; ACT_SITE 561; /note=Charge relay system; /evidence=ECO:0000269|PubMed:17027507; ACT_SITE 679; /note=Charge relay system; /evidence=ECO:0000269|PubMed:17027507 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Selective cleavage of Arg-|-Ser bond in complement component C3 alpha-chain to form C3a and C3b, and Arg-|-Xaa bond in complement component C5 alpha-chain to form C5a and C5b.; EC=3.4.21.43; |
| DNA Binding | |
| EC Number | 3.4.21.43 |
| Enzyme Function | FUNCTION: Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor C4b to generate the C3 or C5 convertase. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (3); Beta strand (43); Chain (3); Disulfide bond (8); Domain (5); Glycosylation (8); Helix (24); Metal binding (3); Motif (1); Natural variant (6); Sequence conflict (5); Signal peptide (1); Turn (3) |
| Keywords | 3D-structure;Age-related macular degeneration;Alternative splicing;Complement pathway;Direct protein sequencing;Disease variant;Disulfide bond;Glycoprotein;Hydrolase;Immunity;Innate immunity;Metal-binding;Protease;Reference proteome;Repeat;Secreted;Serine protease;Signal;Sushi |
| Interact With | O43889-2; Q15125; Q9Y282; Q5JX71; P15941-11; Q9BY50; Q14973; Q9NQQ7-3; P27105 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence="ECO:0000269|PubMed:10734221, ECO:0000269|PubMed:6922702" |
| Structure 3D | X-ray crystallography (5) |
| Cross Reference PDB | 2I6Q; 2I6S; 2ODP; 2ODQ; 3ERB; |
| Mapped Pubmed ID | 10531307; 10925294; 11694537; 12396008; 12823772; 12878586; 15199963; 16670774; 17182573; 17234210; 17576744; 18069935; 18174230; 18493315; 18515590; 18541650; 18806293; 18806297; 18936151; 18987644; 19015224; 19043567; 19117936; 19169232; 19180512; 19197355; 19259132; 19336475; 19344414; 19399715; 19423540; 19556007; 19661236; 19696172; 19851445; 19913121; 19948975; 20157618; 20205591; 20378180; 20381870; 20385819; 20406964; 20417301; 20438785; 20523265; 20628086; 20679960; 20711500; 20801516; 20888482; 21881993; 22232432; 22273503; 22610944; 22869612; 23060141; 23233260; 23313922; 2420889; 24453474; 24965207; 24983375; 25732348; 26176736; 27252379; 28742139; 29283494; 293688; 293746; 30179527; 32164349; 32913345; 6019133; 6238120; 6906228; 70787; 7086154; 8786315; 978134; |
| Motif | MOTIF 260..264; /note=MIDAS-like motif |
| Gene Encoded By | |
| Mass | 83,268 |
| Kinetics | |
| Metal Binding | METAL 262; /note=Divalent metal cation; METAL 264; /note=Divalent metal cation; METAL 337; /note=Divalent metal cation |
| Rhea ID | |
| Cross Reference Brenda |