Detail Information for IndEnz0002017369
IED ID IndEnz0002017369
Enzyme Type ID protease017369
Protein Name ATP-dependent Clp protease proteolytic subunit 2
EC 3.4.21.92
Endopeptidase Clp 2
Gene Name clpP2 DSY3354
Organism Desulfitobacterium hafniense (strain Y51)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Firmicutes Clostridia Eubacteriales Peptococcaceae Desulfitobacterium Desulfitobacterium hafniense (Desulfitobacterium frappieri) Desulfitobacterium hafniense (strain Y51)
Enzyme Sequence MSYLVPMVIEQTNRGERSYDIYSRLLKDRIIFLGGPVTDDVANLVVAQMLFLEAEDPEKDIFLYINSPGGSISAGMAIYDTMQYIRADVHTICVGLAASMGAFLLTAGAKGKRQALPNAEILIHQPLIGGGGISGQATEIEIHAKHLLRVKERMNRILAERTGQTIERIEADTDRDRYMTAEEAKEYGLIDEVLEKPNQPKK
Enzyme Length 202
Uniprot Accession Number Q24S49
Absorption
Active Site ACT_SITE 99; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_00444; ACT_SITE 124; /evidence=ECO:0000255|HAMAP-Rule:MF_00444
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).; EC=3.4.21.92; Evidence={ECO:0000255|HAMAP-Rule:MF_00444};
DNA Binding
EC Number 3.4.21.92
Enzyme Function FUNCTION: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins. {ECO:0000255|HAMAP-Rule:MF_00444}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1)
Keywords Cytoplasm;Hydrolase;Protease;Reference proteome;Serine protease
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00444}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 22,421
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda