Detail Information for IndEnz0002017438
IED ID IndEnz0002017438
Enzyme Type ID protease017438
Protein Name ATP-dependent Clp protease proteolytic subunit 2
EC 3.4.21.92
Endopeptidase Clp 2
Gene Name clpP2 pc1376
Organism Protochlamydia amoebophila (strain UWE25)
Taxonomic Lineage cellular organisms Bacteria PVC group Chlamydiae Chlamydiia Parachlamydiales Parachlamydiaceae Candidatus Protochlamydia Candidatus Protochlamydia amoebophila Protochlamydia amoebophila (strain UWE25)
Enzyme Sequence MSHSSHPRPEMSLTPYVIEDTGRGERSMDIYSRLLKDRIIFIGTEITDQVANVVVAQLLFLKMEDPKKDVHIYINSLGGYITSGLAIYDTMQYLGCDINTYCIGQAASMGALLLTAGTKGKRFALPNSRIMIHQPYGGVGGTSADVALQAKEILELKKRLNHIMADSTRQPLEKIAADSERDFYMSAVEAKDYGLIDEVVVPKKKTT
Enzyme Length 207
Uniprot Accession Number Q6MBE9
Absorption
Active Site ACT_SITE 108; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_00444; ACT_SITE 133; /evidence=ECO:0000255|HAMAP-Rule:MF_00444
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).; EC=3.4.21.92; Evidence={ECO:0000255|HAMAP-Rule:MF_00444};
DNA Binding
EC Number 3.4.21.92
Enzyme Function FUNCTION: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins. {ECO:0000255|HAMAP-Rule:MF_00444}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1)
Keywords Cytoplasm;Hydrolase;Protease;Reference proteome;Serine protease
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00444}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 22,903
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda