Detail Information for IndEnz0002017776
IED ID IndEnz0002017776
Enzyme Type ID protease017776
Protein Name ATP-dependent Clp protease ATP-binding subunit ClpX
Gene Name clpX BSU28220
Organism Bacillus subtilis (strain 168)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis (strain 168)
Enzyme Sequence MFKFNEEKGQLKCSFCGKTQDQVRKLVAGPGVYICDECIELCTEIVEEELGTEEEVEFKDVPKPQEIREILNEYVIGQDQAKKSLAVAVYNHYKRINSNSKVDDVELSKSNISLIGPTGSGKTLLAQTLARILNVPFAIADATSLTEAGYVGEDVENILLKLIQAADYDVEKAEKGIIYIDEIDKVARKSENPSITRDVSGEGVQQALLKILEGTVASVPPQGGRKHPHQEFIQIDTTNILFICGGAFDGIEQIIKRRLGQKVIGFGADNKAADLEKEDLLSKVLPEDLLRFGLIPEFIGRLPVIASLEKLDEEALVAILTKPKNALVKQFKKMLELDNVELEFEEEALSEIAKKAIERKTGARGLRSIIEGIMLDVMFELPSRDDIEKCVITGATVTHGEPPRLLLKDGTEVSQDKTSA
Enzyme Length 420
Uniprot Accession Number P50866
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP (By similarity). Probably the major protease that degrades proteins tagged by trans-translation (PubMed:11395451). {ECO:0000255|HAMAP-Rule:MF_00175, ECO:0000269|PubMed:11395451}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding NP_BIND 117..124; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_00175
Features Chain (1); Domain (1); Metal binding (4); Nucleotide binding (1); Sequence conflict (10)
Keywords ATP-binding;Chaperone;Metal-binding;Nucleotide-binding;Reference proteome;Stress response;Zinc
Interact With
Induction INDUCTION: By heat shock. {ECO:0000269|PubMed:8973311}.
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 22512862;
Motif
Gene Encoded By
Mass 46,350
Kinetics
Metal Binding METAL 13; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU01250; METAL 16; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU01250; METAL 35; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU01250; METAL 38; /note=Zinc; /evidence=ECO:0000255|PROSITE-ProRule:PRU01250
Rhea ID
Cross Reference Brenda