| IED ID | IndEnz0002017939 |
| Enzyme Type ID | protease017939 |
| Protein Name |
ATP-dependent Clp protease proteolytic subunit EC 3.4.21.92 Endopeptidase Clp |
| Gene Name | clpP HDEF_0809 |
| Organism | Hamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Enterobacteriaceae incertae sedis ant tsetse mealybug aphid etc. endosymbionts aphid secondary symbionts Candidatus Hamiltonella Candidatus Hamiltonella defensa Hamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT) |
| Enzyme Sequence | MSSFDEQNTRLPSMTLVPMVVEQTSRGERAYDIYSRMLKERVIFLTGQVEEHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFITPDVSTICIGQACSMGAFLLAAGAKSKRYCLPNARVMIHQPLGGFQGQASDIEIHAKEILKVKKRMNDLMAIHTKKTLKIIEKDTERDHFLSAEEAKKYGLVDCVLTERKDVKNFSKK |
| Enzyme Length | 215 |
| Uniprot Accession Number | C4K4P2 |
| Absorption | |
| Active Site | ACT_SITE 111; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_00444; ACT_SITE 136; /evidence=ECO:0000255|HAMAP-Rule:MF_00444 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).; EC=3.4.21.92; Evidence={ECO:0000255|HAMAP-Rule:MF_00444}; |
| DNA Binding | |
| EC Number | 3.4.21.92 |
| Enzyme Function | FUNCTION: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins. {ECO:0000255|HAMAP-Rule:MF_00444}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1) |
| Keywords | Cytoplasm;Hydrolase;Protease;Reference proteome;Serine protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00444}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 24,259 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |