Detail Information for IndEnz0002018037
IED ID IndEnz0002018037
Enzyme Type ID protease018037
Protein Name ATP-dependent Clp protease proteolytic subunit
EC 3.4.21.92
Endopeptidase Clp
Gene Name clpP LCA_0531
Organism Latilactobacillus sakei subsp. sakei (strain 23K) (Lactobacillus sakei subsp. sakei)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Lactobacillales Lactobacillaceae Latilactobacillus Latilactobacillus sakei (Lactobacillus sakei) Latilactobacillus sakei subsp. sakei Latilactobacillus sakei subsp. sakei (strain 23K) (Lactobacillus sakei subsp. sakei)
Enzyme Sequence MLVPTVIEQTSRGERAYDIYSRLLKDRIIMLSGEVNDQMANTIIAQLLFLDAQDSDKDISIYINSPGGSVTAGLAIMDTMNFIKSDVQTIAMGMAASMASVLLSAGTKGKRFALPNSTVLIHQPLGGAQGQQTEIEIAAREILKTRKRLNQILADNSGQTFEKLQADTDRDNYMTAQEAKDYGLIDDIMVNQPK
Enzyme Length 194
Uniprot Accession Number Q38Y96
Absorption
Active Site ACT_SITE 97; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_00444; ACT_SITE 122; /evidence=ECO:0000255|HAMAP-Rule:MF_00444
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).; EC=3.4.21.92; Evidence={ECO:0000255|HAMAP-Rule:MF_00444};
DNA Binding
EC Number 3.4.21.92
Enzyme Function FUNCTION: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins. {ECO:0000255|HAMAP-Rule:MF_00444}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1)
Keywords Cytoplasm;Hydrolase;Protease;Reference proteome;Serine protease
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00444}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 21,258
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda