Detail Information for IndEnz0002018207
IED ID IndEnz0002018207
Enzyme Type ID protease018207
Protein Name Cartilage intermediate layer protein 1
CILP-1

Cleaved into: Cartilage intermediate layer protein 1 C1; Cartilage intermediate layer protein 1 C2
Gene Name Cilp
Organism Mus musculus (Mouse)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse)
Enzyme Sequence MAAIKTWVFSFLVLEVTTVLGRQTMLAQSVRRVQPVKRTPKTLAKPADSQESPGEWTTWFNIDHPGGQGDYERLDAIRFYYGERVCARPLRLEARTTDWMPAGSTGQVVHGSPREGFWCLNREQRPGQNCSNYTVRFLCPPGSLRGDAEHIWSSWSPWSKCSAACGHTGVQTRTRTCLAQTVSLCSEATEEGQLCMSQACTACDLTCPMGQVNADCDACMCQDFMLHGAISLPGGGPAPGAAVYLLAKAPKMLTRTDSSGRFRVPGLCPDGKTILKITKTKFAPIMITMPKTSLKSATINAEFVRAETPYIVMNPEMKARRAGQSVSLCCKATGKPSPDKYFWYHNNTLLDPSLYKHESKLVLRNLQQDQAGEYFCKAQSDAGAVKSKVTQLTVIAHDETPCNPTPESYLIRLPHDCFQNASNSFYYDVGRCPIKTCAGQQDNGIRCRDAVENCCGISRTEEREIQCSGYTLPTKVAVECSCQRCAETRSIVRGRVTATDNGEPMRFGHVYMGNNRVSMTGYKGTFTLHIPQDTERLVLTFVDRLQKFVNTTKVLPFNKKGSAVFHEIKMLRQKEPITLEAMETNIIPLGEVIGEDPVAELEIPSKSFYRQNGEPFTGKVKASVTFLDPRNISTATAAQSDLNFINDEGDTFPLRTYGMFSVDFRDEATSESLNAGKVKVHLDSTQVKMPEHVPAMKLWSLNPDTGLWEEEGDFKFESQRRNKREERTFLVGNMEIRERRLFNLDVPESRRCFIKVRTYRSERFLPSEQIQGVVVSVINLEPRTGFSSNPRAWGRFDSVITGPNGACLPAFCDDQSPDAYSVYVLASLSGEELEAVESSPKFNPNAIGVPQPYLNKLKYRRTDHEDPRVKKTAFQISMAKPRPNSAEESNGPIYAFENLRACEEAPPSAAHFRFYQIEGDRYDYNTVPFNEDDPMSWTEDYLAWWPKPMEFRACYIKVKIVGPLEVNVRSRNMGGTHRQTVGKLYGIRDVKSTRDRDQPNVSSACLEFKCSGMLYDQDRVDRTLVKVIPQGSCHRASVNSMLHEYLVNHLPLAVNNDTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAKEIALGRCFDGTSDGSSRIMKSNVGVALTFNCAERQVGRQSAFQYLQSTPARSPATGTVQGRVPAMRQQRASRGGLRRRGSMAPLRFSGVAQQPLSN
Enzyme Length 1184
Uniprot Accession Number Q66K08
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (3); Disulfide bond (4); Domain (2); Frameshift (1); Glycosylation (8); Region (1); Sequence conflict (4); Signal peptide (1)
Keywords Cleavage on pair of basic residues;Disulfide bond;Extracellular matrix;Glycoprotein;Immunoglobulin domain;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix {ECO:0000250}.
Modified Residue
Post Translational Modification PTM: Cleaved into 2 chains possibly by a furin-like protease upon or preceding secretion. {ECO:0000250}.
Signal Peptide SIGNAL 1..21; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 12466851; 16140986; 21267068; 21677750; 24194600; 24443808; 24631904; 29438665; 33903225; 34610755;
Motif
Gene Encoded By
Mass 132,334
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda