Detail Information for IndEnz0002018252
IED ID IndEnz0002018252
Enzyme Type ID protease018252
Protein Name ATP-dependent Clp protease proteolytic subunit
EC 3.4.21.92
Endopeptidase Clp
Gene Name clpP A1G_04200
Organism Rickettsia rickettsii (strain Sheila Smith)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Alphaproteobacteria Rickettsiales Rickettsiaceae Rickettsieae Rickettsia spotted fever group Rickettsia rickettsii Rickettsia rickettsii (strain Sheila Smith)
Enzyme Sequence MSYVPIVIEPTSRGERAYDIYSRLLKERIIFVCSTVEDHMANLIVAQLLFLEAENPKKDIYMYINSPGGVVTAGLAIYDTMQYIKPKVATLCIGQACSMGSLLLCGGEKGMRYSLPHSRIMIHQPSGGYKGQATDIEIHAQETLKIKRLLNELYSKHTEQELQHIEKSMERDNFMSPEEAKKFGLVDNIISSRDVMALLAK
Enzyme Length 201
Uniprot Accession Number A8GSH5
Absorption
Active Site ACT_SITE 98; /note=Nucleophile; /evidence=ECO:0000255|HAMAP-Rule:MF_00444; ACT_SITE 123; /evidence=ECO:0000255|HAMAP-Rule:MF_00444
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).; EC=3.4.21.92; Evidence={ECO:0000255|HAMAP-Rule:MF_00444};
DNA Binding
EC Number 3.4.21.92
Enzyme Function FUNCTION: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins. {ECO:0000255|HAMAP-Rule:MF_00444}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1)
Keywords Cytoplasm;Hydrolase;Protease;Serine protease
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00444}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 22,644
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda