Detail Information for IndEnz0002018284
IED ID IndEnz0002018284
Enzyme Type ID protease018284
Protein Name Calcium-activated chloride channel regulator 1
EC 3.4.-.-
Calcium-activated chloride channel
Epithelial chloride channel protein
Gene Name
Organism Bos taurus (Bovine)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Ruminantia Pecora Bovidae Bovinae Bos (oxen cattle) Bos taurus (Bovine)
Enzyme Sequence MVPRLTVILFLTLHLLPGMKSSMVNLINNGYDGIVIAINPSVPEDEKLIQNIKEMVTEASTYLFHATKRRVYFRNVSILIPMTWKSKSEYLMPKQESYDQAEVIVANPYLKHGDDPYTLQYGRCGEKGQYIHFTPNFLLTNNLPIYGSRGRAFVHEWAHLRWGIFDEYNGDQPFYISRRNTIEATRCSTHITGTNVIVKCQGGSCITRPCRRDSQTGLYEAKCTFIPEKSQTARESIMFMQSLHSVTEFCTEKTHNVEAPNLQNKMCNGKSTWDVIMNSTDFQNTSPMTEMNPPTQPTFSLLKSKQRVVCLVLDKSGSMSSEDRLFRMNQAAELFLIQIIEKGSLVGMVTFDSVAEIRNNLTKITDDNVYENITANLPQEANGGTSICRGLKAGFQAIIQSQQSTSGSEIILLTDGEDNEIHSCIEEVKQSGVIIHTIALGPSAAKELETLSDMTGGHRFYANKDINGLTNAFSRISSRSGSITQQTIQLESKALAITEKKWVNGTVPVDSTIGNDTFFVVTWTIKKPEILLQDPKGKKYKTSDFKEDKLNIHSARLRIPGIAETGTWTYSLLNNHASPQILTVTVTTRARSPTTPPVTATAHMSQNTAHYPSPVIVYAQVSQGFLPVLGINVTAIIETEDGHQVTLELWDNGAGADTVKNDGIYSRYFTDYRGNGRYSLKVHAEARNNTARLSLRQPQNKALYIPGYIENGKIILNPPRPEVKDDLAKAEIEDFSRLTSGGSFTVSGAPPGNHPSVLPPNKIIDLEAKFKEDHIQLSWTAPANVLDKGKANSYIIRISKSFLDLQKDFDNATLVNTSSLKPKEAGSDENFEFKPEPFRIENGTNFYIAVQAINEANLTSEVSNIAQAIKFIPMPEDSVPALGTKISAINLAIFALAMILSIV
Enzyme Length 903
Uniprot Accession Number P54281
Absorption
Active Site ACT_SITE 156; /evidence=ECO:0000250|UniProtKB:A8K7I4
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.-.-
Enzyme Function FUNCTION: May be involved in mediating calcium-activated chloride conductance (PubMed:8537359). May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC. {ECO:0000250|UniProtKB:A8K7I4, ECO:0000269|PubMed:8537359}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Domain (1); Glycosylation (4); Metal binding (3); Region (1); Signal peptide (1); Transmembrane (1)
Keywords Calcium;Calcium transport;Cell membrane;Chloride;Glycoprotein;Hydrolase;Ion transport;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport;Zinc
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Apical cell membrane {ECO:0000269|PubMed:8537359}; Single-pass membrane protein {ECO:0000255}.
Modified Residue
Post Translational Modification PTM: Glycosylated. {ECO:0000269|PubMed:8537359}.; PTM: The 125-kDa product is autoproteolytically processed by the metalloprotease domain and yields to two cell-surface-associated subunits, a 90-kDa protein and a group of 37- to 41-kDa proteins. The cleavage is necessary for calcium-activated chloride channel (CaCC) activation activity. {ECO:0000250|UniProtKB:A8K7I4}.
Signal Peptide SIGNAL 1..21; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 100,306
Kinetics
Metal Binding METAL 155; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:A8K7I4; METAL 159; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:A8K7I4; METAL 166; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:A8K7I4
Rhea ID
Cross Reference Brenda