| IED ID | IndEnz0002018287 |
| Enzyme Type ID | protease018287 |
| Protein Name |
Chymosin EC 3.4.23.4 Preprorennin |
| Gene Name | CYM CPC |
| Organism | Bos taurus (Bovine) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Ruminantia Pecora Bovidae Bovinae Bos (oxen cattle) Bos taurus (Bovine) |
| Enzyme Sequence | MRCLVVLLAVFALSQGAEITRIPLYKGKSLRKALKEHGLLEDFLQKQQYGISSKYSGFGEVASVPLTNYLDSQYFGKIYLGTPPQEFTVLFDTGSSDFWVPSIYCKSNACKNHQRFDPRKSSTFQNLGKPLSIHYGTGSMQGILGYDTVTVSNIVDIQQTVGLSTQEPGDVFTYAEFDGILGMAYPSLASEYSIPVFDNMMNRHLVAQDLFSVYMDRNGQESMLTLGAIDPSYYTGSLHWVPVTVQQYWQFTVDSVTISGVVVACEGGCQAILDTGTSKLVGPSSDILNIQQAIGATQNQYGEFDIDCDNLSYMPTVVFEINGKMYPLTPSAYTSQDQGFCTSGFQSENHSQKWILGDVFIREYYSVFDRANNLVGLAKAI |
| Enzyme Length | 381 |
| Uniprot Accession Number | P00794 |
| Absorption | |
| Active Site | ACT_SITE 92; /evidence="ECO:0000255|PROSITE-ProRule:PRU10094, ECO:0000269|PubMed:329280, ECO:0000269|PubMed:4612029"; ACT_SITE 274; /evidence="ECO:0000255|PROSITE-ProRule:PRU10094, ECO:0000269|PubMed:329280, ECO:0000269|PubMed:4612029" |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single 104-Ser-Phe-|-Met-Ala-107 bond in kappa-chain of casein.; EC=3.4.23.4; |
| DNA Binding | |
| EC Number | 3.4.23.4 |
| Enzyme Function | FUNCTION: Chymosin is synthesized in the mucosa of the abomasum (fourth stomach) of young (unweaned) ruminants. The enzyme hydrolyzes casein to paracasein. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (27); Chain (1); Disulfide bond (3); Domain (1); Helix (9); Mutagenesis (2); Natural variant (1); Propeptide (1); Sequence conflict (13); Signal peptide (1); Turn (6) |
| Keywords | 3D-structure;Aspartyl protease;Digestion;Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Reference proteome;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..16; /evidence="ECO:0000269|PubMed:240697, ECO:0000269|PubMed:329280, ECO:0000269|PubMed:6283469" |
| Structure 3D | X-ray crystallography (6) |
| Cross Reference PDB | 1CMS; 1CZI; 3CMS; 4AA8; 4AUC; 4CMS; |
| Mapped Pubmed ID | 23633601; 9862200; |
| Motif | |
| Gene Encoded By | |
| Mass | 42,180 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.23.4; |