| IED ID | IndEnz0002018288 |
| Enzyme Type ID | protease018288 |
| Protein Name |
Calcium-activated chloride channel regulator 1 EC 3.4.-.- Calcium-activated chloride channel family member 1 eCLCA1 |
| Gene Name | CLCA1 |
| Organism | Equus caballus (Horse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Perissodactyla (odd-toed ungulates) Equidae (horses) Equus Equus Equus caballus (Horse) |
| Enzyme Sequence | MGSFKSSVFILVLHLLEGALSNSLIHLNNNGYEGIVIAIDPNVPEDETLIQQIKDMVTQASPYLFEATEKRFYFKNVAILVPENWKTKPEYERPKLETYKNADVLVAEPNPPGNDQPYTEQMGKCGEKGERIYFTPDFLAGKRLDEYGPQGRVFVHEWAHLRWGLFNEYNDDQKFYLSNKEIKPVKCSADIAGKNVVNHCQGGSCATKPCRRDRVTGLYAQECEFIPDEQQTEKASIMFSQSIDSVVEFCTEENHNREAPNMQNQRCNLRSTWEVIRDSEDFKKTTPMTAQPPQPTFSLLQIGQRIVCLVLDKSGSMAIGDRLKRLTQAGKLFLLQTVEQGSWVGMVTFDSAAYVQSALRQIKGGTDRDALTKSLPTVASGGTSICSGLRSAFTVIRKKYKTDGSEIVLLTDGEDNTISSCFNEVKQSGAIIHTVALGPSAAAELEELSKMTGGLQTYASDQAQNNGLIDAFGALSSGNGAVSQHSIQLVSRGSTLQNSQWMNGTVIVDSTVGNDTLFLITWTSQPPQILLWDPSGKKQDGFVVDTNTKMAYLQVPGTAKVGTWTYSLQASSQTLTLTVTSRASSATLPPVTVTSKVNKDTGKFPSPVVVYAKIHQGGLPILRATVTALIESVDGKTVTLELLDNGAGADATKDDGIYSRYFTAYNTNGRYSIKVWALGGVNAARQMGISQQNGAMYRAGRMKNGEIIWNPPRPKINIDDLLSKQVPFSRTSSGGSFVASNVPNAPIPDLFPPCQITDLKAKIQGDSLINLTWTAPGDDYDHGRADRYIIRISTNILDLRDKFNDSLQVNTTDLIPKEATSEEVFVFKPENIAFENGTDLFIAIQAVDEVDLESEISNIAQVSLFLPPQTPPETPSPSLPCPDSNITSAIPGIHILKVMWKWLGELQLSVALG |
| Enzyme Length | 913 |
| Uniprot Accession Number | Q2TU62 |
| Absorption | |
| Active Site | ACT_SITE 157; /evidence=ECO:0000250|UniProtKB:A8K7I4 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.-.- |
| Enzyme Function | FUNCTION: May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Domain (1); Glycosylation (7); Metal binding (3); Natural variant (2); Region (1); Sequence conflict (1); Signal peptide (1); Site (1) |
| Keywords | Autocatalytic cleavage;Calcium;Calcium transport;Chloride;Glycoprotein;Hydrolase;Ion transport;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Transport;Zinc |
| Interact With | |
| Induction | INDUCTION: Strongly up-regulated in bronchioles with recurrent airway obstruction (RAO). {ECO:0000269|PubMed:15879574}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | PTM: Glycosylated. {ECO:0000269|PubMed:15879574}.; PTM: The translation product is autoproteolytically cleaved by the metalloprotease domain in the endoplasmic reticulum into a N-terminal and a C-terminal products that remain physically associated with each other. The cleavage is necessary for calcium-activated chloride channel (CaCC) activation activity. {ECO:0000250|UniProtKB:A8K7I4}. |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 100,134 |
| Kinetics | |
| Metal Binding | METAL 156; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:A8K7I4; METAL 160; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:A8K7I4; METAL 167; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:A8K7I4 |
| Rhea ID | |
| Cross Reference Brenda |