Detail Information for IndEnz0002018309
IED ID IndEnz0002018309
Enzyme Type ID protease018309
Protein Name Calcium-activated chloride channel regulator 1
EC 3.4.-.-
Calcium-activated chloride channel family member 3
mCLCA3
Protein gob-5
Gene Name Clca1 Clca3 Gob5
Organism Mus musculus (Mouse)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse)
Enzyme Sequence MESLKSPVFLLILHLLEGVLSESLIQLNNNGYEGIVIAIDHDVPEDEALIQHIKDMVTQASPYLFEATGKRFYFKNVAILIPESWKAKPEYTRPKLETFKNADVLVSTTSPLGNDEPYTEHIGACGEKGIRIHLTPDFLAGKKLTQYGPQDRTFVHEWAHFRWGVFNEYNNDEKFYLSKGKPQAVRCSAAITGKNQVRRCQGGSCITNGKCVIDRVTGLYKDNCVFVPDPHQNEKASIMFNQNINSVVEFCTEKNHNQEAPNDQNQRCNLRSTWEVIQESEDFKQTTPMTAQPPAPTFSLLQIGQRIVCLVLDKSGSMLNDDRLNRMNQASRLFLLQTVEQGSWVGMVTFDSAAYVQSELKQLNSGADRDLLIKHLPTVSAGGTSICSGLRTAFTVIKKKYPTDGSEIVLLTDGEDNTISSCFDLVKQSGAIIHTVALGPAAAKELEQLSKMTGGLQTYSSDQVQNNGLVDAFAALSSGNAAIAQHSIQLESRGVNLQNNQWMNGSVIVDSSVGKDTLFLITWTTHPPTIFIWDPSGVEQNGFILDTTTKVAYLQVPGTAKVGFWKYSIQASSQTLTLTVTSRAASATLPPITVTPVVNKNTGKFPSPVTVYASIRQGASPILRASVTALIESVNGKTVTLELLDNGAGADATKNDGVYSRFFTAFDANGRYSVKIWALGGVTSDRQRAAPPKNRAMYIDGWIEDGEVRMNPPRPETSYVQDKQLCFSRTSSGGSFVATNVPAAAPIPDLFPPCQITDLKASIQGQNLVNLTWTAPGDDYDHGRASNYIIRMSTSIVDLRDHFNTSLQVNTTGLIPKEASSEEIFEFELGGNTFGNGTDIFIAIQAVDKSNLKSEISNIARVSVFIPAQEPPIPEDSTPPCPDISINSTIPGIHVLKIMWKWLGEMQVTLGLH
Enzyme Length 913
Uniprot Accession Number Q9D7Z6
Absorption
Active Site ACT_SITE 157; /evidence=ECO:0000250|UniProtKB:A8K7I4
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.-.-
Enzyme Function FUNCTION: May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC. {ECO:0000269|PubMed:11296262, ECO:0000269|PubMed:11694454, ECO:0000269|PubMed:16645179}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Domain (1); Glycosylation (6); Metal binding (3); Region (1); Sequence conflict (1); Signal peptide (1); Site (1)
Keywords Autocatalytic cleavage;Calcium;Calcium transport;Chloride;Glycoprotein;Hydrolase;Ion transport;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Transport;Zinc
Interact With
Induction INDUCTION: By IL3, IL4 and IL9 in the lung. Increases in the bronchiolar epithelium of asbestos-induced fibrogenesis. Decreases in cystic fibrosis knockout mice. {ECO:0000269|PubMed:11694454, ECO:0000269|PubMed:16099848, ECO:0000269|PubMed:16251409}.
Subcellular Location SUBCELLULAR LOCATION: Secreted, extracellular space {ECO:0000269|PubMed:12019299, ECO:0000269|PubMed:15919655, ECO:0000269|PubMed:16895902}. Note=The 75 kDa N-terminal and a 35 kDa C-terminal products are fully secreted into extracellular environment as a soluble complex of two glycoproteins.
Modified Residue
Post Translational Modification PTM: The 110 kDa translation product is autoproteolytically cleaved by the metalloprotease domain in the endoplasmic reticulum into a 75 kDa N-terminal and a 35 kDa C-terminal products that remain physically associated with each other. The cleavage is necessary for calcium-activated chloride channel (CaCC) activation activity. {ECO:0000250|UniProtKB:A8K7I4, ECO:0000269|PubMed:12019299}.; PTM: Glycosylated. {ECO:0000269|PubMed:12019299, ECO:0000269|PubMed:16895902}.
Signal Peptide SIGNAL 1..21; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 10828589; 11217851; 12466851; 12568493; 15163632; 15947424; 16569774; 16868010; 16936271; 16985003; 17522155; 18923035; 19210762; 20479003; 20819519; 21267068; 22080371; 22362387; 22675208; 23213461; 23266329; 23449471; 23691121; 24033950; 24905173; 25033194; 25855264; 26075897; 26162072; 27010197; 27173853; 27224029; 29610986; 29885864; 31201093; 31570526; 34066250;
Motif
Gene Encoded By
Mass 100,071
Kinetics
Metal Binding METAL 156; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:A8K7I4; METAL 160; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:A8K7I4; METAL 167; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:A8K7I4
Rhea ID
Cross Reference Brenda