Detail Information for IndEnz0002018312
IED ID IndEnz0002018312
Enzyme Type ID protease018312
Protein Name Calcium-activated chloride channel regulator 1
EC 3.4.-.-
Calcium-activated chloride channel family member 1
pCLCA1
Gene Name CLCA1 AECC
Organism Sus scrofa (Pig)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Laurasiatheria Artiodactyla Suina Suidae (pigs) Sus Sus scrofa (Pig)
Enzyme Sequence MGSFRSSLFILVLHLLEGAQSNSLIQLNGNGYEGIVIAIDPNVPEDERLIQNIKDMVTKASPYLFEATEKRFYFKNVAILIPASWKAKPEYVKPKLETYKNADVVVTEPNPPENDGPYTEQMGNCGEKGEKIYFTPDFVAGKKVLQYGPQGRVFVHEWAHLRWGVFNEYNNEQKFYLSNKKEQPVICSAAIRGTNVLPQCQGGSCVTKPCRADRVTGLFQKECEFIPDPQQSEKASIMFAQSIDTVVEFCKEKNHNKEAPNDQNQKCNLRSTWEVIQDSEDFKKTTPMTTQPPAPTFSLLQIGQRIVCLVLDKSGSMTVGGRLKRLNQAGKLFLLQTVEQGAWVGMVAFDSAAYVKSELVQINSAAERDALARSLPTAASGGTSICSGLRSAFTVIKKKYPTDGSEIVLLTDGEDNTISACFPEVKQNGAIIHTVALGPSAAKELEELSQMTGGLQTYASDQAENNGLIDAFGALSSGNRAASQRSIQLESQGLTLQNNEWMNGTVVVDSTVGKDTLFLITLERKFLSPIPFFGVPSGRSQDSFLVGKHNKMAYFQVPGTAKVGMWKYSLQASSQTLTLTVSSRRSSATLPPVTVTSKMNKDTGKFPSPMVVYTKIHQGTLPILRAKVTALIESENGKTVTLELLDNGAGADATKNDGIYSRYFTAYDANGRYSVKVWALGGVNTPRRRAPPLWSGAMYIRGWIENGEIKWNPPRPDINKDDLQGKQVCFSRTASGGSFVASDVPKSPIPDLFPPCKITDLKAGIQGDNLINLTWTAPGDDYDHGRADRYIIRISTNILDLRDKFNDSVQVNTTDLIPKEANSEEVFVFKPEGIPFTNGTDLFIAVQAVDKTNLKSEISNIAQVSLFLPPEAPPETPPETPAPSLPCPEIQVNSTIPGIHILKIMWKWLGELQLSIA
Enzyme Length 917
Uniprot Accession Number Q9TUB5
Absorption
Active Site ACT_SITE 157; /evidence=ECO:0000250|UniProtKB:A8K7I4
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.-.-
Enzyme Function FUNCTION: May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC. Induces a cAMP-dependent chloride conductance possibly through effects on CFTR in colon carcinoma cells. {ECO:0000269|PubMed:11015605, ECO:0000269|PubMed:12107050, ECO:0000269|PubMed:12408984, ECO:0000269|PubMed:14988065}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Domain (1); Glycosylation (6); Metal binding (3); Region (1); Signal peptide (1); Site (1)
Keywords Autocatalytic cleavage;Calcium;Calcium transport;Chloride;Glycoprotein;Hydrolase;Ion transport;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Transport;Zinc
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, extracellular space {ECO:0000250}.
Modified Residue
Post Translational Modification PTM: Glycosylated. {ECO:0000250}.; PTM: The translation product is autoproteolytically cleaved by the metalloprotease domain in the endoplasmic reticulum into a N-terminal and a C-terminal products that remain physically associated with each other. The cleavage is necessary for calcium-activated chloride channel (CaCC) activation activity. {ECO:0000250|UniProtKB:A8K7I4}.
Signal Peptide SIGNAL 1..21; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 19755716;
Motif
Gene Encoded By
Mass 100,736
Kinetics
Metal Binding METAL 156; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:A8K7I4; METAL 160; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:A8K7I4; METAL 167; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:A8K7I4
Rhea ID
Cross Reference Brenda