Detail Information for IndEnz0002018356
IED ID IndEnz0002018356
Enzyme Type ID protease018356
Protein Name Clathrin heavy chain 1
Clathrin heavy chain on chromosome 17
CLH-17
Gene Name CLTC CLH17 CLTCL2 KIAA0034
Organism Homo sapiens (Human)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human)
Enzyme Sequence MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRMAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEVVQAANTSGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVANVELYYRAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKVDKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM
Enzyme Length 1675
Uniprot Accession Number Q00610
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:16968737, PubMed:21297582). The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825). Plays a role in early autophagosome formation (PubMed:20639872). {ECO:0000269|PubMed:15858577, ECO:0000269|PubMed:16968737, ECO:0000269|PubMed:20639872, ECO:0000269|PubMed:21297582, ECO:0000269|PubMed:23532825}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Alternative sequence (2); Beta strand (27); Chain (1); Erroneous initiation (1); Helix (6); Initiator methionine (1); Modified residue (18); Mutagenesis (17); Natural variant (8); Region (16); Repeat (7); Sequence conflict (5); Turn (7)
Keywords 3D-structure;Acetylation;Alternative splicing;Autophagy;Cell cycle;Cell division;Coated pit;Cytoplasm;Cytoplasmic vesicle;Cytoskeleton;Direct protein sequencing;Disease variant;Membrane;Mental retardation;Mitosis;Phosphoprotein;Reference proteome;Repeat
Interact With P09496; P10242; Q01968; Q07912; O75674
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane {ECO:0000269|PubMed:17081065}; Peripheral membrane protein {ECO:0000269|PubMed:17081065}; Cytoplasmic side {ECO:0000269|PubMed:17081065}. Membrane, coated pit {ECO:0000269|PubMed:17081065}; Peripheral membrane protein {ECO:0000269|PubMed:17081065}; Cytoplasmic side {ECO:0000269|PubMed:17081065}. Melanosome {ECO:0000269|PubMed:17081065}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:15858577, ECO:0000269|PubMed:16968737, ECO:0000269|PubMed:23918938}. Note=Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In complex with TACC3 and CKAP5 (forming the TACC3/ch-TOG/clathrin complex) localized to inter-microtubule bridges in mitotic spindles. {ECO:0000269|PubMed:25596274}.
Modified Residue MOD_RES 2; /note="N-acetylalanine"; /evidence="ECO:0000269|Ref.7, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378, ECO:0007744|PubMed:25944712"; MOD_RES 67; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:23186163"; MOD_RES 105; /note="Phosphothreonine"; /evidence="ECO:0000250|UniProtKB:Q68FD5"; MOD_RES 184; /note="Phosphotyrosine"; /evidence="ECO:0000250|UniProtKB:Q68FD5"; MOD_RES 394; /note="Phosphothreonine"; /evidence="ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569"; MOD_RES 634; /note="Phosphotyrosine"; /evidence="ECO:0007744|PubMed:19690332"; MOD_RES 737; /note="N6-succinyllysine"; /evidence="ECO:0000250|UniProtKB:Q68FD5"; MOD_RES 856; /note="N6-acetyllysine"; /evidence="ECO:0007744|PubMed:19608861"; MOD_RES 899; /note="Phosphotyrosine"; /evidence="ECO:0000250|UniProtKB:Q68FD5"; MOD_RES 1167; /note="Phosphoserine"; /evidence="ECO:0000250|UniProtKB:Q68FD5"; MOD_RES 1206; /note="Phosphotyrosine"; /evidence="ECO:0000250|UniProtKB:Q68FD5"; MOD_RES 1229; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:23186163"; MOD_RES 1441; /note="N6-acetyllysine; alternate"; /evidence="ECO:0007744|PubMed:19608861"; MOD_RES 1441; /note="N6-succinyllysine; alternate"; /evidence="ECO:0000250|UniProtKB:Q68FD5"; MOD_RES 1477; /note="Phosphotyrosine"; /evidence="ECO:0007744|PubMed:19690332"; MOD_RES 1487; /note="Phosphotyrosine"; /evidence="ECO:0000250|UniProtKB:Q68FD5"; MOD_RES 1494; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:19369195, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569"; MOD_RES 1501; /note="N6-acetyllysine"; /evidence="ECO:0007744|PubMed:19608861"
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (5)
Cross Reference PDB 2XZG; 4G55; 6E4L; 6QNN; 6QNP;
Mapped Pubmed ID 10089883; 10097102; 10216106; 10394364; 10480952; 10535736; 10542231; 10556217; 10559856; 10559861; 10559924; 10567358; 10593899; 10608864; 10625686; 10702393; 10706082; 10706887; 10747093; 10766823; 10804209; 10818153; 10908605; 10934142; 11007890; 11082044; 11110708; 11157096; 11231140; 11239472; 11280786; 11310834; 11459829; 11470803; 11516654; 11517213; 11577110; 11588219; 11604418; 11751994; 11799118; 11850821; 11877457; 11877461; 11889126; 11919637; 11943732; 11988742; 11994746; 12057195; 12112524; 12213833; 12424201; 12447393; 12717448; 12750159; 12847086; 12920229; 12934099; 12952941; 12952949; 12958364; 12960147; 14521508; 14555962; 14581451; 14596919; 14617351; 14622578; 14622579; 14731593; 14743216; 14962911; 15067034; 15161933; 15208656; 15252009; 15260957; 15318165; 15339664; 15350535; 15351694; 15374880; 15383276; 15494376; 15502812; 15502813; 15556869; 15588951; 15649145; 15703209; 15749704; 15802264; 15895073; 15895077; 15911768; 15916959; 16103228; 16137687; 16138905; 16153455; 16155256; 16161041; 16169070; 16170336; 16215232; 16326391; 16330023; 16410077; 16460808; 16516836; 16618797; 16707488; 16798879; 16825495; 16871283; 16880396; 16902405; 16903783; 16939498; 17018281; 17108328; 17116749; 17158794; 17185414; 17199046; 17220478; 17307723; 17353907; 17353931; 17355222; 17400507; 17452316; 17461779; 17494869; 17500595; 17504809; 17512409; 17540576; 17550971; 17609109; 17620599; 17625570; 17681954; 17762867; 17765681; 17922009; 17952123; 17978091; 18039658; 18042253; 18093523; 18097461; 18199683; 18363968; 18388313; 18457437; 1847920; 18548008; 18593892; 18594010; 18656476; 18701132; 18790740; 18923523; 18923524; 18923525; 18981233; 19064640; 19092055; 19135240; 19287005; 19289168; 19296720; 19367725; 19458185; 19459784; 19463016; 19509056; 19529763; 19536138; 19575674; 19582886; 19603039; 19651769; 19654208; 19696796; 19696798; 19713939; 19738201; 19765186; 19766654; 19776351; 19793827; 19798056; 19805454; 19809571; 19859085; 19910923; 19913121; 20033059; 20059951; 20195357; 20211604; 20231386; 20360068; 20428113; 20448150; 20498856; 20562859; 20566684; 20628086; 20711500; 20826345; 20936779; 20946875; 20947020; 21134980; 2115402; 21164297; 21182205; 21187331; 21213368; 2121367; 21278753; 21316588; 21362119; 21364927; 21415216; 21445324; 21482805; 21494621; 21499258; 21502504; 21508312; 21565175; 21613550; 21656749; 21779028; 21841790; 21883765; 21917865; 21927000; 21927001; 21948233; 21962517; 21965600; 21988832; 22010197; 22099461; 22233676; 22249260; 22321011; 22323290; 22327622; 22335553; 22339630; 22347464; 22357968; 22484487; 22496312; 22505844; 22570254; 22623428; 22658521; 22764087; 22810585; 22815487; 22863004; 22891263; 22935703; 22942912; 22980331; 23027862; 23104988; 23139213; 23201355; 23228632; 23239810; 23297414; 23386615; 23414517; 23416715; 23536704; 23606334; 23661334; 23823722; 23861397; 23861900; 23891661; 23940042; 23957430; 24060861; 24130457; 24186068; 24189400; 24211445; 24384571; 24407285; 24445538; 24475247; 24496003; 24502978; 24518094; 24581490; 24613930; 24675041; 24695225; 24736082; 24746795; 24789820; 24966051; 25107275; 25180771; 25228534; 25241761; 25303366; 25341920; 25342631; 25388970; 25393796; 25421750; 25455218; 25609649; 25631092; 25690657; 25813404; 25852190; 25860340; 26001147; 26042225; 26062823; 26136365; 26301689; 26403691; 26420826; 26464158; 26496610; 26598620; 26638075; 26939704; 26975582; 27245569; 27519163; 27573755; 27874193; 28261837; 28404650; 28659337; 28741662; 29097553; 29455642; 29769406; 29873274; 29973118; 30692217; 31011214; 31366041; 31388026; 31562907; 31776469; 31871319; 31932847; 32737221; 33087446; 33476648; 34436540; 6146630; 7592972; 7650016; 7824924; 7877694; 8374173; 8397411; 8524399; 8552632; 8633037; 8663493; 8670264; 8837779; 8939989; 9092476; 9100026; 9169477; 9202021; 9427626; 9428629; 9497313; 9635431; 9651214; 9723620; 9736607; 9778532;
Motif
Gene Encoded By
Mass 191,615
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda