| IED ID | IndEnz0002018356 |
| Enzyme Type ID | protease018356 |
| Protein Name |
Clathrin heavy chain 1 Clathrin heavy chain on chromosome 17 CLH-17 |
| Gene Name | CLTC CLH17 CLTCL2 KIAA0034 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRMAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEVVQAANTSGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVANVELYYRAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKVDKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM |
| Enzyme Length | 1675 |
| Uniprot Accession Number | Q00610 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:16968737, PubMed:21297582). The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825). Plays a role in early autophagosome formation (PubMed:20639872). {ECO:0000269|PubMed:15858577, ECO:0000269|PubMed:16968737, ECO:0000269|PubMed:20639872, ECO:0000269|PubMed:21297582, ECO:0000269|PubMed:23532825}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (2); Beta strand (27); Chain (1); Erroneous initiation (1); Helix (6); Initiator methionine (1); Modified residue (18); Mutagenesis (17); Natural variant (8); Region (16); Repeat (7); Sequence conflict (5); Turn (7) |
| Keywords | 3D-structure;Acetylation;Alternative splicing;Autophagy;Cell cycle;Cell division;Coated pit;Cytoplasm;Cytoplasmic vesicle;Cytoskeleton;Direct protein sequencing;Disease variant;Membrane;Mental retardation;Mitosis;Phosphoprotein;Reference proteome;Repeat |
| Interact With | P09496; P10242; Q01968; Q07912; O75674 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane {ECO:0000269|PubMed:17081065}; Peripheral membrane protein {ECO:0000269|PubMed:17081065}; Cytoplasmic side {ECO:0000269|PubMed:17081065}. Membrane, coated pit {ECO:0000269|PubMed:17081065}; Peripheral membrane protein {ECO:0000269|PubMed:17081065}; Cytoplasmic side {ECO:0000269|PubMed:17081065}. Melanosome {ECO:0000269|PubMed:17081065}. Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:15858577, ECO:0000269|PubMed:16968737, ECO:0000269|PubMed:23918938}. Note=Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In complex with TACC3 and CKAP5 (forming the TACC3/ch-TOG/clathrin complex) localized to inter-microtubule bridges in mitotic spindles. {ECO:0000269|PubMed:25596274}. |
| Modified Residue | MOD_RES 2; /note="N-acetylalanine"; /evidence="ECO:0000269|Ref.7, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378, ECO:0007744|PubMed:25944712"; MOD_RES 67; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:23186163"; MOD_RES 105; /note="Phosphothreonine"; /evidence="ECO:0000250|UniProtKB:Q68FD5"; MOD_RES 184; /note="Phosphotyrosine"; /evidence="ECO:0000250|UniProtKB:Q68FD5"; MOD_RES 394; /note="Phosphothreonine"; /evidence="ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569"; MOD_RES 634; /note="Phosphotyrosine"; /evidence="ECO:0007744|PubMed:19690332"; MOD_RES 737; /note="N6-succinyllysine"; /evidence="ECO:0000250|UniProtKB:Q68FD5"; MOD_RES 856; /note="N6-acetyllysine"; /evidence="ECO:0007744|PubMed:19608861"; MOD_RES 899; /note="Phosphotyrosine"; /evidence="ECO:0000250|UniProtKB:Q68FD5"; MOD_RES 1167; /note="Phosphoserine"; /evidence="ECO:0000250|UniProtKB:Q68FD5"; MOD_RES 1206; /note="Phosphotyrosine"; /evidence="ECO:0000250|UniProtKB:Q68FD5"; MOD_RES 1229; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:23186163"; MOD_RES 1441; /note="N6-acetyllysine; alternate"; /evidence="ECO:0007744|PubMed:19608861"; MOD_RES 1441; /note="N6-succinyllysine; alternate"; /evidence="ECO:0000250|UniProtKB:Q68FD5"; MOD_RES 1477; /note="Phosphotyrosine"; /evidence="ECO:0007744|PubMed:19690332"; MOD_RES 1487; /note="Phosphotyrosine"; /evidence="ECO:0000250|UniProtKB:Q68FD5"; MOD_RES 1494; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:19369195, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569"; MOD_RES 1501; /note="N6-acetyllysine"; /evidence="ECO:0007744|PubMed:19608861" |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (5) |
| Cross Reference PDB | 2XZG; 4G55; 6E4L; 6QNN; 6QNP; |
| Mapped Pubmed ID | 10089883; 10097102; 10216106; 10394364; 10480952; 10535736; 10542231; 10556217; 10559856; 10559861; 10559924; 10567358; 10593899; 10608864; 10625686; 10702393; 10706082; 10706887; 10747093; 10766823; 10804209; 10818153; 10908605; 10934142; 11007890; 11082044; 11110708; 11157096; 11231140; 11239472; 11280786; 11310834; 11459829; 11470803; 11516654; 11517213; 11577110; 11588219; 11604418; 11751994; 11799118; 11850821; 11877457; 11877461; 11889126; 11919637; 11943732; 11988742; 11994746; 12057195; 12112524; 12213833; 12424201; 12447393; 12717448; 12750159; 12847086; 12920229; 12934099; 12952941; 12952949; 12958364; 12960147; 14521508; 14555962; 14581451; 14596919; 14617351; 14622578; 14622579; 14731593; 14743216; 14962911; 15067034; 15161933; 15208656; 15252009; 15260957; 15318165; 15339664; 15350535; 15351694; 15374880; 15383276; 15494376; 15502812; 15502813; 15556869; 15588951; 15649145; 15703209; 15749704; 15802264; 15895073; 15895077; 15911768; 15916959; 16103228; 16137687; 16138905; 16153455; 16155256; 16161041; 16169070; 16170336; 16215232; 16326391; 16330023; 16410077; 16460808; 16516836; 16618797; 16707488; 16798879; 16825495; 16871283; 16880396; 16902405; 16903783; 16939498; 17018281; 17108328; 17116749; 17158794; 17185414; 17199046; 17220478; 17307723; 17353907; 17353931; 17355222; 17400507; 17452316; 17461779; 17494869; 17500595; 17504809; 17512409; 17540576; 17550971; 17609109; 17620599; 17625570; 17681954; 17762867; 17765681; 17922009; 17952123; 17978091; 18039658; 18042253; 18093523; 18097461; 18199683; 18363968; 18388313; 18457437; 1847920; 18548008; 18593892; 18594010; 18656476; 18701132; 18790740; 18923523; 18923524; 18923525; 18981233; 19064640; 19092055; 19135240; 19287005; 19289168; 19296720; 19367725; 19458185; 19459784; 19463016; 19509056; 19529763; 19536138; 19575674; 19582886; 19603039; 19651769; 19654208; 19696796; 19696798; 19713939; 19738201; 19765186; 19766654; 19776351; 19793827; 19798056; 19805454; 19809571; 19859085; 19910923; 19913121; 20033059; 20059951; 20195357; 20211604; 20231386; 20360068; 20428113; 20448150; 20498856; 20562859; 20566684; 20628086; 20711500; 20826345; 20936779; 20946875; 20947020; 21134980; 2115402; 21164297; 21182205; 21187331; 21213368; 2121367; 21278753; 21316588; 21362119; 21364927; 21415216; 21445324; 21482805; 21494621; 21499258; 21502504; 21508312; 21565175; 21613550; 21656749; 21779028; 21841790; 21883765; 21917865; 21927000; 21927001; 21948233; 21962517; 21965600; 21988832; 22010197; 22099461; 22233676; 22249260; 22321011; 22323290; 22327622; 22335553; 22339630; 22347464; 22357968; 22484487; 22496312; 22505844; 22570254; 22623428; 22658521; 22764087; 22810585; 22815487; 22863004; 22891263; 22935703; 22942912; 22980331; 23027862; 23104988; 23139213; 23201355; 23228632; 23239810; 23297414; 23386615; 23414517; 23416715; 23536704; 23606334; 23661334; 23823722; 23861397; 23861900; 23891661; 23940042; 23957430; 24060861; 24130457; 24186068; 24189400; 24211445; 24384571; 24407285; 24445538; 24475247; 24496003; 24502978; 24518094; 24581490; 24613930; 24675041; 24695225; 24736082; 24746795; 24789820; 24966051; 25107275; 25180771; 25228534; 25241761; 25303366; 25341920; 25342631; 25388970; 25393796; 25421750; 25455218; 25609649; 25631092; 25690657; 25813404; 25852190; 25860340; 26001147; 26042225; 26062823; 26136365; 26301689; 26403691; 26420826; 26464158; 26496610; 26598620; 26638075; 26939704; 26975582; 27245569; 27519163; 27573755; 27874193; 28261837; 28404650; 28659337; 28741662; 29097553; 29455642; 29769406; 29873274; 29973118; 30692217; 31011214; 31366041; 31388026; 31562907; 31776469; 31871319; 31932847; 32737221; 33087446; 33476648; 34436540; 6146630; 7592972; 7650016; 7824924; 7877694; 8374173; 8397411; 8524399; 8552632; 8633037; 8663493; 8670264; 8837779; 8939989; 9092476; 9100026; 9169477; 9202021; 9427626; 9428629; 9497313; 9635431; 9651214; 9723620; 9736607; 9778532; |
| Motif | |
| Gene Encoded By | |
| Mass | 191,615 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |