Detail Information for IndEnz0002018368
IED ID IndEnz0002018368
Enzyme Type ID protease018368
Protein Name ATP-dependent Clp protease proteolytic subunit 4, chloroplastic
EC 3.4.21.92
Endopeptidase ClpP4
nClpP4
nClpP3
Gene Name CLPP4 NCLPP3 NCLPP4 At5g45390 MFC19.5
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MGTLSLSSSLKPSLVSSRLNSSSSASSSSFPKPNNLYLKPTKLISPPLRTTSPSPLRFANASIEMSQTQESAIRGAESDVMGLLLRERIVFLGSSIDDFVADAIMSQLLLLDAKDPKKDIKLFINSPGGSLSATMAIYDVVQLVRADVSTIALGIAASTASIILGAGTKGKRFAMPNTRIMIHQPLGGASGQAIDVEIQAKEVMHNKNNVTSIIAGCTSRSFEQVLKDIDRDRYMSPIEAVEYGLIDGVIDGDSIIPLEPVPDRVKPRVNYEEISKDPMKFLTPEIPDDEIY
Enzyme Length 292
Uniprot Accession Number Q94B60
Absorption
Active Site ACT_SITE 158; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 183; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).; EC=3.4.21.92;
DNA Binding
EC Number 3.4.21.92
Enzyme Function FUNCTION: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity). Essential protein required for chloroplast development and integrity (PubMed:16705403, PubMed:17241447). Essential for Embryogenesis (PubMed:23548781). {ECO:0000250, ECO:0000269|PubMed:16705403, ECO:0000269|PubMed:17241447, ECO:0000269|PubMed:23548781}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Erroneous gene model prediction (1); Erroneous initiation (2); Modified residue (1); Sequence conflict (5); Transit peptide (1)
Keywords Acetylation;Chloroplast;Direct protein sequencing;Hydrolase;Plastid;Protease;Reference proteome;Serine protease;Transit peptide;Ubl conjugation
Interact With
Induction INDUCTION: Repressed in darkness. Levels decrease in leaves during aging (at protein level). Slightly and transiently repressed by high light stress (at protein level). {ECO:0000269|PubMed:10427773, ECO:0000269|PubMed:11982939, ECO:0000269|PubMed:16705403}.
Subcellular Location SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000269|PubMed:11982939, ECO:0000269|PubMed:14593120}.
Modified Residue MOD_RES 66; /note=N-acetylserine; /evidence=ECO:0007744|PubMed:22223895
Post Translational Modification PTM: Ubiquitinated by CHIP. {ECO:0000269|PubMed:17241447}.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 12185496; 12938931; 14576160; 15028209; 16207701; 16548064; 16640601; 16648217; 16895613; 17181860; 18431481; 18633119; 21166475; 24529374; 26085677; 27247031; 28627464; 29155321;
Motif
Gene Encoded By
Mass 31,498
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda