Detail Information for IndEnz0002018377
IED ID IndEnz0002018377
Enzyme Type ID protease018377
Protein Name ATP-dependent Clp protease proteolytic subunit 6, chloroplastic
EC 3.4.21.92
Endopeptidase ClpP6
nClpP6
nClpP1
Gene Name CLPP6 NCLPP1 NCLPP6 At1g11750 F25C20.10
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MAGLAISPPLGLSFSSRTRNPKPTSFLSHNQRNPIRRIVSALQSPYGDSLKAGLSSNVSGSPIKIDNKAPRFGVIEAKKGNPPVMPSVMTPGGPLDLSSVLFRNRIIFIGQPINAQVAQRVISQLVTLASIDDKSDILMYLNCPGGSTYSVLAIYDCMSWIKPKVGTVAFGVAASQGALLLAGGEKGMRYAMPNTRVMIHQPQTGCGGHVEDVRRQVNEAIEARQKIDRMYAAFTGQPLEKVQQYTERDRFLSASEALEFGLIDGLLETEY
Enzyme Length 271
Uniprot Accession Number Q9SAA2
Absorption
Active Site ACT_SITE 175; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 200; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).; EC=3.4.21.92;
DNA Binding
EC Number 3.4.21.92
Enzyme Function FUNCTION: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity). Essential protein required for chloroplast development and integrity. {ECO:0000250, ECO:0000269|PubMed:16980539}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Compositional bias (1); Region (1); Sequence conflict (4); Transit peptide (1)
Keywords Alternative splicing;Chloroplast;Direct protein sequencing;Hydrolase;Plastid;Protease;Reference proteome;Serine protease;Transit peptide
Interact With
Induction INDUCTION: Repressed in darkness. Induced during cold acclimation (at protein level). {ECO:0000269|PubMed:10427773, ECO:0000269|PubMed:11982939}.
Subcellular Location SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000269|PubMed:11982939, ECO:0000269|PubMed:14593120, ECO:0000269|PubMed:9951729}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 29,382
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda