Detail Information for IndEnz0002018378
IED ID IndEnz0002018378
Enzyme Type ID protease018378
Protein Name Probable ATP-dependent Clp protease proteolytic subunit
EC 3.4.21.92
Endopeptidase Clp
Fragments
Gene Name
Organism Populus euphratica (Euphrates poplar)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Malpighiales Salicaceae Saliceae Populus (poplars) Populus euphratica (Euphrates poplar)
Enzyme Sequence IALQSPAGAARIRDYLYNELSK
Enzyme Length 22
Uniprot Accession Number P84982
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).; EC=3.4.21.92; Evidence={ECO:0000250|UniProtKB:Q3MDK7, ECO:0000255|PROSITE-ProRule:PRU10085, ECO:0000255|PROSITE-ProRule:PRU10086};
DNA Binding
EC Number 3.4.21.92
Enzyme Function FUNCTION: Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity). {ECO:0000250|UniProtKB:Q3MDK7}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Non-adjacent residues (1); Non-terminal residue (2)
Keywords Direct protein sequencing;Hydrolase;Protease;Serine protease
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 2,450
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda