| IED ID | IndEnz0002018477 |
| Enzyme Type ID | protease018477 |
| Protein Name |
Guanylate cyclase alpha PfGCalpha EC 4.6.1.2 Guanylyl cyclase |
| Gene Name | GCalpha PF3D7_1138400 |
| Organism | Plasmodium falciparum (isolate 3D7) |
| Taxonomic Lineage | cellular organisms Eukaryota Sar Alveolata Apicomplexa Aconoidasida Haemosporida (haemosporidians) Plasmodiidae Plasmodium Plasmodium (Laverania) Plasmodium falciparum Plasmodium falciparum (isolate 3D7) |
| Enzyme Sequence | MSDSKKHYNESNVRYKVKYSGKIFGNNMKKLNENNNHDFTTAEAAWMEKKKKLYKDTNNQPLWDFRKIQINPTETDELISFPSNKICSKNYGKYSFIFKGLYEQFLRLPNIWFLLISLLEFIPQYQNLSNYMYYSKHSSFFLLLFFICVSIIKNIYEDSRRSNIDYQINNRLCHMLDGPNSQLKAVRWMELSVGSIIRLIENEQVPADILLLSCNNSEGVVYIETSLLNGETNLNKKYCVNETRNETSIYAISNIRGRIVCEKPNSNMESFNGSLKLDAHPRATSLSINNVIFKGSHIKNTEYIFGVILYTGNDTKIMKNISNNKHKLGYVNKELNSYTIIGLIFTFICVFISVLFKWTEDDKFRNGSHFFLITVKDNICESIVKYTLLYSNIIPISILISVDLISILQSILIENDNHISTFENYETSEPSTIDDMDNELGDFKMDKSHTFFKKYTYFLNNRSKNFTNNRYSSTNTERASDFRKSFFGTFDKLKTIKRYFYSIKSKIKSISQSNTLYNKSSAGGSIIRSDKNKQTTFSKSFIDIFQRTNNNEHSQTLDNNNNNDNNNNNNICKEKYQNVNNSKNVYVSEDSSNNKYKQNDYLQKSQEQNEIHSINNNNYNNKNDNNLQTEFSNHNFRINAYKTNRTTKDDTYNNKHISLNSRSKSNQKGILINSNEINRKKKKNLLQKIFPFFKKKRTNISEDLSKYVFRRSKQLNINHNNSEKYNLNDYDEYGWGLCLNSNMHGDLGNVDFIFTDKTGTLTNNNMTFNMCSIAGKTYGSKCKNKKKIYNNPKSNNDNKLNSYDKNIFKNKFSSEASIKKISFVRDFSNNVSSKNDLTLDDPTELISDEGNNYLRDKYEHTSDKKNDTNKNRDGANNSNNNNNKDVSNNKNKNNNNYNYNSNNDYIKQNGECNIYNNQNNTNNTHNNNIYYDEYNNTNEGNMKNTNVNKDTNSKCNKKDFIINTSDIINNNNNNNNDQKTNNLKYKSSSSWNSKAKKSSYINLNSYNKYQSFQNSSMLSSSLSSTCSSETEDENGSQLMYYSSSNYDNLDINNDMDSTKTYSHYTEQNYYNSVKDDKDINSEHQRYQQKKTNKQTKNIQQNQCDQWEDNRYTQESIKNQYFFDRNYQTFLMKQNFPRPKSFRLNMNSSSVKGRCDFNDTKIYNDLNSQSWRGYYIDEFFKCMTLCHAVTPFIQFDYIRNKHLTYTNSRSSKGISNNMDRYNINPTTTTTNNNNNNNNINNINNINNNNNNNNNNNNNKQFSCDYMKNRNDIIFETSTDYYANQDNAKRKREKTLFDNVSHCGEIQEYRDNKKYRMKRSQTCSNNRKIFSNQRTLYDYRNMNNNLKNKNSYLRNKLKSKIFLDKSYRKRKKSSGTYKGSDICKGIEKKETRRWKRFLHMKGSNNRNIFSRIKDFKSNSIIYNNQDKTNDYKNGYSNNINNNNSNNYTNEKINRSFNKRHSAVSFKQMSKEKLIPINNVLSNRCDVESYPNEELSFSKKKKKNSEIVSFASGKSDIGFEERTDAGIAGKNMELSYNELEKYKYVKHIDSNNKYINNNNNSIHMSHMSSNNNTFESHIYFDAIKYQSSSLDEECLIYSSSFLGYRLVLRNKNTMCIEIDGSFNKWTIIGVNEFTNRRGKMSIVVKPDSMESGSILYVKGSDSSILSLLNLKYSKFLDKKYSRKRRNKNIQKYKQKREEYNELKSKDINIEEFQKSKSLSLKNFKNNTPKVLFGECTLDNNNNNINKNYKYDKNDKHNNNNNNNNNNSNYSYNYLHDSNNLDAMQNQKYSYAYEDKEDYYYTNNGECNKYKERYRRLEKQLRKFSVKGLRSMIFAFRYLSEEETIKYKRMYDDACSSIYNKEQRLEKVAEEFERDLIYLGITGVKNGLQEKVPKTIDILNQSGIRIWMLTGDNVEYSLHVSFLCKFLNKHTKIFHAALENSNAKKLKREGMALYELFQLEKEEKKPYENLCLLVNGRNLQTFLNYTDLQTHFLNMACTCDVVIACRITAKQKAFLVQLIKNRLYPTPNTLAIGDGANDIAMIQEANIGVSIMTSDCIISAGYSDYCIKKFCYLRKLLFIYGSKHLYTISIILYWNFFKNILLILPIFFYQAYASWSCVKIYPELLYTFFSIFWVFIPIIYYMFLQHNLNYDILYNIPLFYALSRRRYNMNCFKFLPWIFEAIFYSMIIYFFAYAALKENSHLNNGEVITINTFGNICFIGCLLISILRLFLEGSLWSPSILITCFGCFLFVFFPSLLFICFAYLSNEYIREVFRQTFLWAPLYVLLILWFSTCIISYIFINFTKSILFPNIYNVVNHWLFEQYQEKHNKNKYIFSLSGKNKFLKLRKLGKKIKFKFKRNYSKKYDTNVIREHPLLHHTFKSEQDQNKCNEQFSKDPFISNSLLKSKNCKFKKDSSYSKSNIHVDEEQTYKGLKVVLSEQVVHAKENLVSFKNEKKNKNNKNTSMNVNDNIIIKNNNINIKNNDNNNDDNDNDNNNNNNNNDNYNNNDHNKKEFKQKHIQNNYENLFNHTQVENNLIKENLSIRNDNNRRDNKNETDYIKQSDEDYEMNPSITSLRKRECINDSKYIDNKSYDNKISNNHEEKDGFKSDDRSLINSNMMDYKNEFTENDTTYSFNDSHMFYDFPSNSKTTKDYKVINSRIDDSIMADMKNCLNPLKNTFLVDLLPPGKRFRINEDHIYFKNNERMENIPEALDVNKKYYHIIQNKAEYYDNDSLSEFSYTSSNSSINNKKKNENTQKEIVKVSHLINRFTLAFKDMQLESGFQIHKKNKFYKTFTPWYRFIFLLLGVFFLYVWKLESSLSQLWNMPSDASTDVFILFLSLLLELVLLAATVTTFFSNIFIENFNKIISAVVILIITYHVVSYSVTHIDGVFQAVLFPLYTFVILRLPFVNAVLCNIIFLGLFIIRFNGDHFLDKKGLAHYIPLFIGVDVFVGFVGYRLEYNQRKNFLLEYSVESSRRKQREILNTMLPPFVVDEMIYSELNEEGIPISLKAEDISTVTIIFCDIYDFQNIVASIEPTRLVEVLDRLFLCFDKCTEQFNCTKIETVFETYLAACGLVKREKDEDELENNKYSNNNKNNNNNYYYNRKKKKKKNNNNNNNNNNNNNNNNNNNNNLNNNNNNNNVNTSDDDGDFFEEDDANNHQIYNQMKGQEDAHDSIDFALSILHVSSHIKYEKSKMMLKKNDSFEDANDDTHNVNDSFNNDKAENDNTNTDNNKPTRIRVKVGIHSGRIIAGVVGSKKPQYALFGDTVNTASRMKTTGKPDYIHISEATYNLVKDDKTLIYEKKETEIKGKGIMTTYLLTSVIGLNYPFLGEHVEKKGHFISELYEDDLSNNLNYDKTQNILGYYSNKINQNNDSNINEGIINNNMNTFNTIDGDTSNYYVNLKDLSLNDLPNEYIKDVNTHYCEIIKLRNLKIGTAVGYQLKQKDFYNMTFLNNGLNNDTATNFNQYIESHKDFDDIKDLRIGMNKLSISDSLYYLNENVIRNEPINEYKNKIKINSVNTNKKSYEDAHLNAGNIIYTNNEYPNGQNIEEDDDLLLTNHYNKTNVNNNNNNNIYDVSKELIEHNEEDYKNILMCSKRCKNCNETYCTPNDCNNNDHVYHVMYHRKLSNIKKNYLKNIRKDANIKKDIEKEELNKIHSNKKSFNFFYLFSYIFKIFPFIGKINKEEKKNKSYKKGEQDKSSKRIIALNSEWIFLKFSDKNLEAKYRAHFYSNKSNINSIEQALIIFLVTFVMQTLISSTVSIVFIDHKRATQTLHINYFAYWSVRSVYTFFGFVLWLLFHYRTRPEVSSLLNIKWMIFFLNLLFISAACVFSIAYLWAISETDQTTSYTIWMTNDTIEFFFYLVILHHNTGMLFQTCILVDLLFITMSLTFIATSVVKTITTDSTVLLIPWYVAFNLISTYCKESIDRRTFYANESAKKTENRATELLNDMLPKHVLEEFQQDKLKLAYTHDRLTFLFADICGFTSWANGVDASEVLTLLQKLFAKFDNDSTKYGLYKLCTIGDAYVAISEPVTEDNKDYDPVDGTERVLEMAYSMIRIIKEIREKLYIPNLNMRIGLHYGSCVGGVIGSGRLRYDLWGIDVLTGNLMESNGIPGKINVSETLKNFLLQQFKNRFIFKPHTTIRVIYKDVKCFIITDKKEVESSNHSKLLQNKNYLLNKKFSTQNYLVKNIFAKKKHSSISSSKNIHSYDEKKKKKNDLFYINVNDRQSNL |
| Enzyme Length | 4226 |
| Uniprot Accession Number | Q8IHY1 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=GTP = 3',5'-cyclic GMP + diphosphate; Xref=Rhea:RHEA:13665, ChEBI:CHEBI:33019, ChEBI:CHEBI:37565, ChEBI:CHEBI:57746; EC=4.6.1.2; Evidence={ECO:0000305|PubMed:33500341}; |
| DNA Binding | |
| EC Number | 4.6.1.2 |
| Enzyme Function | FUNCTION: Catalyzes the synthesis of the second messenger cGMP from GTP (Probable). In asexual blood stage schizonts, required for cGMP production which is essential for PKG activation, PKG-dependent Ca(2+) release, and ultimately merozoite egress from host erythrocytes (PubMed:33500341). {ECO:0000269|PubMed:33500341, ECO:0000305|PubMed:33500341}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (5); Domain (2); Glycosylation (2); Metal binding (5); Mutagenesis (1); Region (7); Sequence conflict (11); Topological domain (23); Transmembrane (22) |
| Keywords | Cell membrane;Cytoplasmic vesicle;Glycoprotein;Lyase;Magnesium;Membrane;Metal-binding;Reference proteome;Transmembrane;Transmembrane helix;cGMP biosynthesis |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:10747978}; Multi-pass membrane protein {ECO:0000255}. Cytoplasmic vesicle membrane {ECO:0000269|PubMed:33500341}; Multi-pass membrane protein {ECO:0000255}. Note=In gametocytes, localizes to the cell membrane and/or the parasitophorous vacuole membrane. {ECO:0000269|PubMed:10747978}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 496,021 |
| Kinetics | |
| Metal Binding | METAL 3975; /note=Magnesium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00099; METAL 3975; /note=Magnesium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00099; METAL 3976; /note=Magnesium 2; via carbonyl oxygen; /evidence=ECO:0000255|PROSITE-ProRule:PRU00099; METAL 4019; /note=Magnesium 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00099; METAL 4019; /note=Magnesium 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00099 |
| Rhea ID | RHEA:13665 |
| Cross Reference Brenda |