| IED ID | IndEnz0002018481 |
| Enzyme Type ID | protease018481 |
| Protein Name |
Anion/proton exchange transporter GEF1 CLC protein GEF1 ClC-A ClC-Y1 Voltage-gated chloride channel Cleaved into: GEF1 N-terminal; GEF1 C-terminal |
| Gene Name | GEF1 CLCY1 YJR040W J1616 |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MPTTYVPINQPIGDGEDVIDTNRFTNIPETQNFDQFVTIDKIAEENRPLSVDSDREFLNSKYRHYREVIWDRAKTFITLSSTAIVIGCIAGFLQVFTETLVNWKTGHCQRNWLLNKSFCCNGVVNEVTSTSNLLLKRQEFECEAQGLWIAWKGHVSPFIIFMLLSVLFALISTLLVKYVAPMATGSGISEIKVWVSGFEYNKEFLGFLTLVIKSVALPLAISSGLSVGKEGPSVHYATCCGYLLTKWLLRDTLTYSSQYEYITAASGAGVAVAFGAPIGGVLFGLEEIASANRFNSSTLWKSYYVALVAITTLKYIDPFRNGRVILFNVTYDRDWKVQEIPIFIALGIFGGLYGKYISKWNINFIHFRKMYLSSWPVQEVLFLATLTALISYFNEFLKLDMTESMGILFHECVKNDNTSTFSHRLCQLDENTHAFEFLKIFTSLCFATVIRALLVVVSYGARVPAGIFVPSMAVGATFGRAVSLLVERFISGPSVITPGAYAFLGAAATLSGITNLTLTVVVIMFELTGAFMYIIPLMIVVAITRIILSTSGISGGIADQMIMVNGFPYLEDEQDEEEEETLEKYTAEQLMSSKLITINETIYLSELESLLYDSASEYSVHGFPITKDEDKFEKEKRCIGYVLKRHLASKIMMQSVNSTKAQTTLVYFNKSNEELGHRENCIGFKDIMNESPISVKKAVPVTLLFRMFKELGCKTIIVEESGILKGLVTAKDILRFKRIKYREVHGAKFTYNEALDRRCWSVIHFIIKRFTTNRNGNVI |
| Enzyme Length | 779 |
| Uniprot Accession Number | P37020 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Anion/proton exchange transporter involved in iron and copper cation homeostasis. Involved in intracellular iron metabolism during growth on fermentable and non fermentable carbon sources. Required for proper copper-loading and maturation of multicopper oxidase FET3. Important for adjusting intracellular compartment pH to more alkaline pH under iron limitation. May also transport chloride ions through the plasma membrane. {ECO:0000269|PubMed:12074596, ECO:0000269|PubMed:17662057, ECO:0000269|PubMed:7505388, ECO:0000269|PubMed:9520490, ECO:0000269|PubMed:9614122}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (3); Domain (2); Sequence conflict (5); Site (3); Topological domain (13); Transmembrane (12) |
| Keywords | CBS domain;Chloride;Chloride channel;Endosome;Golgi apparatus;Ion channel;Ion transport;Membrane;Reference proteome;Repeat;Transmembrane;Transmembrane helix;Transport |
| Interact With | Q12154 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000269|PubMed:9614122}; Multi-pass membrane protein {ECO:0000255}. Endosome membrane {ECO:0000305|PubMed:9520490}; Multi-pass membrane protein {ECO:0000255}. Prevacuolar compartment membrane {ECO:0000269|PubMed:12074596}; Multi-pass membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | PTM: Proteolytically processed in the secretory pathway by protease KEX2 within the first extracellular loop. However, both the N- and C-terminal products of the cleavage reaction are required for assembly of a functional channel. {ECO:0000269|PubMed:15710404}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10082675; 10395584; 10748251; 11102523; 11283351; 12114553; 15766533; 16093310; 16775381; 17601488; 17872921; 18378800; 18467557; 19536198; 20530571; 21479832; 21908599; 22222113; 23192874; 23202731; 24040173; 26246599; 27504114; 7648364; 7887248; 8969232; 9207144; 9712830; 9811853; |
| Motif | |
| Gene Encoded By | |
| Mass | 87,592 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |