| IED ID | IndEnz0002018484 |
| Enzyme Type ID | protease018484 |
| Protein Name |
Probable glutamate carboxypeptidase AMP1 EC 3.4.17.21 Probable glutamate carboxypeptidase 2 Probable glutamate carboxypeptidase II Protein ALTERED MERISTEM PROGRAM 1 AtAMP1 Protein CONSTITUTIVE MORPHOGENESIS 2 Protein HAUPTLING Protein MULTIFOLIA Protein PRIMORDIA TIMING |
| Gene Name | AMP1 COP2 HPT MFO1 PT At3g54720 T5N23_80 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MSQPLTTRPTVTGISIIPFRQPPPLCSFLFVIVLFVATFYTLHHPDAVTPPLLFSRNAYNALRLRRLFLSSASNATISSYLRELTRHPHLAGTKPSLDTLHYVFNHFQSLGLETHVAEYEALLSYPTHISVTASFSNTTTLEFDLNDVPGDSPVVRPYHAYSPSGSAQGNVVFVNHGEERDYHALESIGVSVKGCVVLARKGENLGRGAIVKIAEAKGALGVLIYAENDGGGFGGIERGTVMRGIGDPVSPGWPGVVGGEKLSLDDELVTRRFPKIPSLPLSLRNAEIILASLGGARAPLEWRNSGRVGPGQRVGPGRMVINMTFQGEMKMKKINNVVVTIRGSEEADRYVILGNHRDAWTYGAVDPNSGTSALLDISRRFALLLKSGWRPRRTILLCSWDAEEFGMIGSTEWIEENVLNLGASAVAYLNVDCAVQGSGFFAGATPQLDGLLVDVLKLVQDPDAVGLTVEETFKSQNNIIQRLSRVDSDFSGFLHHAGIPSIDMYYGADYPVYHTAFDSYDWMIHNADPLFHRHVAMAGIWGLLGILLADEPLIPFDYISYADQLQAHRDKLSKLLEGKVSVNPLSMAIQEFSLVAKEAADEAKKLKGKSYSKNDVAAAAKRRELNDRLMLVERGFLDAEGIKGKEWFKHLVYGPAAEPESKLGFFPGIADAIAMNASEGIIEHEIWRVARAIQRASKALKGGFT |
| Enzyme Length | 705 |
| Uniprot Accession Number | Q9M1S8 |
| Absorption | |
| Active Site | ACT_SITE 403; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q04609 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.; EC=3.4.17.21; Evidence={ECO:0000305}; |
| DNA Binding | |
| EC Number | 3.4.17.21 |
| Enzyme Function | FUNCTION: May modulate the level of one or more small signaling molecules that have a role in regulating meristem function. May play a role in balancing and restricting the meristem-promoting activity of auxin signaling (PubMed:17553903). Involved in ethylene and giberellin (GA) signaling pathways or in a parallel pathway controlling cell and hypocotyl elongation and cellular organization (PubMed:17006669). Involved in abscisic acid (ABA) signaling pathway. Plays a negative role in ABA-mediated seed germination and seedling development (PubMed:23603279). Acts in association with LAMP1 to suppress ectopic stem cell niche formation in the shoot apical meristem (SAM) independently of cytokinin signaling pathway (PubMed:25673776). Modulates responses to ABA, oxidative stress and abotic stress (PubMed:23621575). Acts as negative regulator of the ABA signaling pathway to modulate freezing and drought stress responses. Mediates carbon and amino acid metabolism (PubMed:23621575, PubMed:23603279). May be involved in the acquisition and/or maintenance of seed dormancy (PubMed:21637772). Involved in the regulation of response to heat shock and plant defense (PubMed:27743891). {ECO:0000269|PubMed:17006669, ECO:0000269|PubMed:17553903, ECO:0000269|PubMed:21637772, ECO:0000269|PubMed:23603279, ECO:0000269|PubMed:23621575, ECO:0000269|PubMed:25673776, ECO:0000269|PubMed:27743891}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Erroneous gene model prediction (1); Glycosylation (4); Metal binding (6); Mutagenesis (2); Region (1); Sequence conflict (1); Topological domain (2); Transmembrane (1) |
| Keywords | Carboxypeptidase;Endoplasmic reticulum;Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Signal-anchor;Transmembrane;Transmembrane helix;Zinc |
| Interact With | O04379 |
| Induction | INDUCTION: Down-regulated by abscisic acid (ABA). {ECO:0000269|PubMed:23603279, ECO:0000269|PubMed:23621575}. |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305|PubMed:25673776}; Single-pass type II membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10409511; 10417706; 11302841; 12271066; 15851402; 16024589; 18203869; 18515128; 18650403; 19597310; 20226671; 23319631; 23622241; 24100209; 25544612; 27208298; 27246880; 28321665; 29884678; 31334862; 31823020; 32198155; 32960882; 8953766; 9294189; 9611173; |
| Motif | |
| Gene Encoded By | |
| Mass | 77,153 |
| Kinetics | |
| Metal Binding | METAL 356; /note=Zinc 1; via tele nitrogen; catalytic; /evidence=ECO:0000250|UniProtKB:Q04609; METAL 366; /note=Zinc 1; catalytic; /evidence=ECO:0000250|UniProtKB:Q04609; METAL 366; /note=Zinc 2; /evidence=ECO:0000250|UniProtKB:Q04609; METAL 404; /note=Zinc 2; /evidence=ECO:0000250|UniProtKB:Q04609; METAL 432; /note=Zinc 1; catalytic; /evidence=ECO:0000250|UniProtKB:Q04609; METAL 514; /note=Zinc 2; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:Q04609 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.17.21; |