| IED ID | IndEnz0002018488 |
| Enzyme Type ID | protease018488 |
| Protein Name |
Gamma-glutamyl peptidase 1 EC 3.4.19.16 |
| Gene Name | GGP1 At4g30530 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MVEQKRYALFLATLDSEFVKKTYGGYHNVFVTTFGDEGEHWDSFRVVSGEFPDEKDLEKYDGFVISGSSHDAFENDDWILKLCDIVKKIDEMKKKILGICFGHQIIARVRGGTVGRAKKGPELKLGDITIVKDAITPGSYFGNEIPDSIAIIKCHQDEVLVLPETAKVLAYSKNYEVEMYSIEDHLFCIQGHPEYNKEILFEIVDRVLALGYVKQEFADAAKATMENRGADRKLWETICKNFLKGRVPTN |
| Enzyme Length | 250 |
| Uniprot Accession Number | Q9M0A7 |
| Absorption | |
| Active Site | ACT_SITE 100; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU00605; ACT_SITE 192; /evidence=ECO:0000255|PROSITE-ProRule:PRU00605; ACT_SITE 194; /evidence=ECO:0000255|PROSITE-ProRule:PRU00605 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=an S-[(1E)-1-(hydroxyimino)-omega-(methylsulfanyl)alkyl]-L-glutathione + H2O = an S-[(1E)-1-(hydroxyimino)-omega-(methylsulfanyl)alkyl]-L-cysteinylglycine + L-glutamate; Xref=Rhea:RHEA:52756, Rhea:RHEA-COMP:13138, Rhea:RHEA-COMP:13355, ChEBI:CHEBI:15377, ChEBI:CHEBI:29985, ChEBI:CHEBI:136061, ChEBI:CHEBI:136825; EC=3.4.19.16; Evidence={ECO:0000269|PubMed:21712415}; CATALYTIC ACTIVITY: Reaction=(E)-1-(glutathione-S-yl)-2-(1H-indol-3-yl)acetohydroximate + H2O = (E)-1-(glycyl-L-cystein-S-yl)-2-(1H-indol-3-yl)acetohydroximate + L-glutamate; Xref=Rhea:RHEA:52764, ChEBI:CHEBI:15377, ChEBI:CHEBI:29985, ChEBI:CHEBI:136444, ChEBI:CHEBI:136831; EC=3.4.19.16; Evidence={ECO:0000269|PubMed:21712415}; CATALYTIC ACTIVITY: Reaction=2-(glutathion-S-yl)-2-(1H-indol-3-yl)acetonitrile + H2O = 2-(glycyl-L-cystein-S-yl)-2-(1H-indol-3-yl)acetonitrile + L-glutamate; Xref=Rhea:RHEA:52768, ChEBI:CHEBI:15377, ChEBI:CHEBI:29985, ChEBI:CHEBI:64981, ChEBI:CHEBI:136832; EC=3.4.19.16; Evidence={ECO:0000269|PubMed:21712415}; CATALYTIC ACTIVITY: Reaction=(Z)-1-(glutathione-S-yl)-2-phenylacetohydroximate + H2O = (Z)-1-(glycyl-L-cystein-S-yl)-2-phenylacetohydroximate + L-glutamate; Xref=Rhea:RHEA:52760, ChEBI:CHEBI:15377, ChEBI:CHEBI:29985, ChEBI:CHEBI:136447, ChEBI:CHEBI:136830; EC=3.4.19.16; Evidence={ECO:0000269|PubMed:21712415}; |
| DNA Binding | |
| EC Number | 3.4.19.16 |
| Enzyme Function | FUNCTION: Involved in glucosinolate biosynthesis. Hydrolyzes the gamma-glutamyl peptide bond of several glutathione (GSH) conjugates to produce Cys-Gly conjugates related to glucosinolates. The gamma-Glu-Cys-Gly-GSH conjugates are the sulfur-donating molecule in glucosinolate biosynthesis (PubMed:19483696, PubMed:21712415). Converts phenylacetohydroximoyl-GSH to benzylglucosinolate (PubMed:19483696). Can use the GSH conjugate of the camalexin intermediate IAN (GS-IAN) as substrate. Required for the biosynthesis of camalexin, a pathogen-inducible phytoalexin with antibacterial and antifungal properties (PubMed:21712415). {ECO:0000269|PubMed:19483696, ECO:0000269|PubMed:21712415}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Secondary metabolite biosynthesis. {ECO:0000305}. |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (1); Sequence conflict (1) |
| Keywords | Cytoplasm;Hydrolase;Protease;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:21712415}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15618427; 16254930; 16552493; 16972165; 18467456; 18854047; 21281472; 23449503; 26106402; 27247031; 28627464; |
| Motif | |
| Gene Encoded By | |
| Mass | 28,385 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=37 uM for phenylacetohydroximoyl-glutathione {ECO:0000269|PubMed:19483696}; |
| Metal Binding | |
| Rhea ID | RHEA:52756; RHEA:52764; RHEA:52768; RHEA:52760 |
| Cross Reference Brenda | 3.4.19.16; |