IED ID | IndEnz0002018490 |
Enzyme Type ID | protease018490 |
Protein Name |
Gamma-glutamyl peptidase 2 EC 3.4.19.16 |
Gene Name | GGP2 At4g30540 |
Organism | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
Enzyme Sequence | MVKQIRRYALFQATPDSEFVKEMYGGYFNVFVSAFGDEGEQWDLFRVIDGEFPRDEDLEKYEGFVISGSLHDAFTEEDWIIELCSVCKKLDVMKKKILGICFGHQIICRVRGGKVGRARKGPDIGLGNITIVQDVIKPGDYFDQIESLSIIQCHRDEVLEPPESARVIGFSDKCDVEIFSVEDHLLCFQGHPEYNKEILLEIIDRVHKIKFVEEEILEKAKDSIKKFEPDTQRLHMLCKNFLKGRRTH |
Enzyme Length | 248 |
Uniprot Accession Number | Q9M0A6 |
Absorption | |
Active Site | ACT_SITE 101; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU00605; ACT_SITE 191; /evidence=ECO:0000255|PROSITE-ProRule:PRU00605; ACT_SITE 193; /evidence=ECO:0000255|PROSITE-ProRule:PRU00605 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=an S-[(1E)-1-(hydroxyimino)-omega-(methylsulfanyl)alkyl]-L-glutathione + H2O = an S-[(1E)-1-(hydroxyimino)-omega-(methylsulfanyl)alkyl]-L-cysteinylglycine + L-glutamate; Xref=Rhea:RHEA:52756, Rhea:RHEA-COMP:13138, Rhea:RHEA-COMP:13355, ChEBI:CHEBI:15377, ChEBI:CHEBI:29985, ChEBI:CHEBI:136061, ChEBI:CHEBI:136825; EC=3.4.19.16; Evidence={ECO:0000250|UniProtKB:Q9M0A7}; CATALYTIC ACTIVITY: Reaction=(E)-1-(glutathione-S-yl)-2-(1H-indol-3-yl)acetohydroximate + H2O = (E)-1-(glycyl-L-cystein-S-yl)-2-(1H-indol-3-yl)acetohydroximate + L-glutamate; Xref=Rhea:RHEA:52764, ChEBI:CHEBI:15377, ChEBI:CHEBI:29985, ChEBI:CHEBI:136444, ChEBI:CHEBI:136831; EC=3.4.19.16; Evidence={ECO:0000250|UniProtKB:Q9M0A7}; CATALYTIC ACTIVITY: Reaction=2-(glutathion-S-yl)-2-(1H-indol-3-yl)acetonitrile + H2O = 2-(glycyl-L-cystein-S-yl)-2-(1H-indol-3-yl)acetonitrile + L-glutamate; Xref=Rhea:RHEA:52768, ChEBI:CHEBI:15377, ChEBI:CHEBI:29985, ChEBI:CHEBI:64981, ChEBI:CHEBI:136832; EC=3.4.19.16; Evidence={ECO:0000250|UniProtKB:Q9M0A7}; CATALYTIC ACTIVITY: Reaction=(Z)-1-(glutathione-S-yl)-2-phenylacetohydroximate + H2O = (Z)-1-(glycyl-L-cystein-S-yl)-2-phenylacetohydroximate + L-glutamate; Xref=Rhea:RHEA:52760, ChEBI:CHEBI:15377, ChEBI:CHEBI:29985, ChEBI:CHEBI:136447, ChEBI:CHEBI:136830; EC=3.4.19.16; Evidence={ECO:0000250|UniProtKB:Q9M0A7}; |
DNA Binding | |
EC Number | 3.4.19.16 |
Enzyme Function | FUNCTION: Involved in glucosinolate biosynthesis. Hydrolyzes the gamma-glutamyl peptide bond of several glutathione (GSH) conjugates to produce Cys-Gly conjugates related to glucosinolates. The gamma-Glu-Cys-Gly-GSH conjugates are the sulfur-donating molecule in glucosinolate biosynthesis. {ECO:0000250|UniProtKB:Q9M0A7}. |
Temperature Dependency | |
PH Dependency | |
Pathway | PATHWAY: Secondary metabolite biosynthesis. {ECO:0000305}. |
nucleotide Binding | |
Features | Active site (3); Chain (1); Domain (1) |
Keywords | Cytoplasm;Hydrolase;Protease;Reference proteome |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9M0A7}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | 17559508; 17915010; |
Motif | |
Gene Encoded By | |
Mass | 28,695 |
Kinetics | |
Metal Binding | |
Rhea ID | RHEA:52756; RHEA:52764; RHEA:52768; RHEA:52760 |
Cross Reference Brenda |