Detail Information for IndEnz0002018493
IED ID IndEnz0002018493
Enzyme Type ID protease018493
Protein Name Gamma-glutamyl peptidase 5
EC 3.4.19.-
Gene Name GGP5 At2g23970
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MVNEQKRFALFLATSDSTFVKKAYGGYFNVFVSTFGEDGEQWDLFRVIDGEFPDDKDLDKYDGFVISGSLNDAFGDDDWIVKLCSLCQKLDDMKKKVLGICFGHQILSRIKGGKVGRASRGLDMGLRSITMVTDAVKPGGYFGSQIPKSLAIIKCHQDEVLELPESATLLAYSDKYNVEMCSYGNHLLGIQGHPEYNKEILFEIIDRVVNLKLMEQDFADKAKATMENAEPDRKQWQTLCKNFLKGRSEQV
Enzyme Length 251
Uniprot Accession Number O82225
Absorption
Active Site ACT_SITE 101; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU00605; ACT_SITE 193; /evidence=ECO:0000255|PROSITE-ProRule:PRU00605; ACT_SITE 195; /evidence=ECO:0000255|PROSITE-ProRule:PRU00605
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.19.-
Enzyme Function FUNCTION: Involved in glucosinolate biosynthesis. Hydrolyzes the gamma-glutamyl peptide bond of several glutathione (GSH) conjugates to produce Cys-Gly conjugates related to glucosinolates. The gamma-Glu-Cys-Gly-GSH conjugates are the sulfur-donating molecule in glucosinolate biosynthesis. {ECO:0000250|UniProtKB:Q9M0A7}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Secondary metabolite biosynthesis. {ECO:0000305}.
nucleotide Binding
Features Active site (3); Chain (1); Domain (1)
Keywords Cytoplasm;Hydrolase;Protease;Reference proteome
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9M0A7}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 16299181; 18775970; 20226671; 23858430;
Motif
Gene Encoded By
Mass 28,312
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda