Detail Information for IndEnz0002018496
IED ID IndEnz0002018496
Enzyme Type ID protease018496
Protein Name Gliomedin
Cleaved into: Gliomedin shedded ectodomain
Gene Name Gldn
Organism Rattus norvegicus (Rat)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat)
Enzyme Sequence MTRAAERGQGATGWGLRGALMAVALLSVLNAVGTVFVLYQWRELSAALRALEAQHGQEQREDSALRAFLAELSRAPARVPEPPQDPMSAARNKRSHGGEPASHIRAESQDMMMMMTYSMVPIRVMIDLCNSTQGICLTGPPGPPGPPGAGGLPGHNGSDGQPGLQGPKGEKGAVGKRGKMGLPGATGNPGEKGEKGDAGELGLPGNEGPPGQKGDKGDKGDVSNDVLLTGAKGDQGPPGPPGPPGPPGPPGSRRAKGPRQPNSFTNQCPGETCVIPNDDTLVGRADEKVNERHSPQTEPMITSIGNPAQVLKVKETFGTWLRESANRSDDRIWVTEHFSGIMVKEFEDLPALLNSSFTLLHLPHYFHGCGHAVYNNSLYYHKGGSNTIVRFEFGKETPQTLKLEDALYFDRKYLFANSKTYFNIAVDEKGLWIIYASSVDGSSILVAQLDERTFSVLRHINTTYPKSKAGNAFIAQGILYVTDTKDTRVTFAFDLLRGKQINANFGLRMSQSVLAMLSYNMRDQHLYSWEDGHLMLYPVHFSSTAPSQR
Enzyme Length 549
Uniprot Accession Number Q80WL1
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Ligand for NRCAM and NFASC/neurofascin that plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Mediates interaction between Schwann cell microvilli and axons via its interactions with NRCAM and NFASC (PubMed:16039564). Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters. Required, together with NRCAM, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier (By similarity). {ECO:0000250|UniProtKB:Q8BMF8, ECO:0000269|PubMed:16039564}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Beta strand (19); Chain (2); Compositional bias (2); Domain (3); Glycosylation (6); Helix (3); Mutagenesis (2); Region (2); Site (2); Topological domain (2); Transmembrane (1); Turn (5)
Keywords 3D-structure;Cell membrane;Cell projection;Collagen;Developmental protein;Differentiation;Direct protein sequencing;Extracellular matrix;Glycoprotein;Membrane;Neurogenesis;Reference proteome;Repeat;Secreted;Signal-anchor;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:16039564}; Single-pass type II membrane protein {ECO:0000269|PubMed:16039564}. Cell projection, axon {ECO:0000269|PubMed:16039564, ECO:0000269|PubMed:22009740}. Note=Detected at the nodes of Ranvier (PubMed:16039564, PubMed:22009740). Detected at immature heminodes (PubMed:22009740). {ECO:0000269|PubMed:16039564, ECO:0000269|PubMed:22009740}.; SUBCELLULAR LOCATION: [Gliomedin shedded ectodomain]: Secreted {ECO:0000269|PubMed:17485493}. Secreted, extracellular space, extracellular matrix {ECO:0000269|PubMed:17485493}. Note=Proteolytic processing gives rise to a soluble extracellular domain that is secreted. The gliomedin shedded ectodomain localizes to the nodes of Ranvier. {ECO:0000269|PubMed:17485493}.
Modified Residue
Post Translational Modification PTM: N-glycosylated. {ECO:0000269|PubMed:17485493}.; PTM: Proteolytic proccessing by a furin-like protease causes shedding of the ectodomain (PubMed:17485493). Further cleavage by BMP1 releases the olfactomedin-like domain. {ECO:0000250|UniProtKB:Q8BMF8, ECO:0000269|PubMed:17485493}.
Signal Peptide
Structure 3D X-ray crystallography (2)
Cross Reference PDB 4D77; 4D7C;
Mapped Pubmed ID 22885108; 25372825;
Motif
Gene Encoded By
Mass 59,330
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda