| IED ID | IndEnz0002018497 |
| Enzyme Type ID | protease018497 |
| Protein Name |
Autolysin EC 3.4.24.38 Gamete lytic enzyme GLE Gametolysin |
| Gene Name | |
| Organism | Chlamydomonas reinhardtii (Chlamydomonas smithii) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Chlorophyta core chlorophytes Chlorophyceae CS clade Chlamydomonadales Chlamydomonadaceae Chlamydomonas Chlamydomonas reinhardtii (Chlamydomonas smithii) |
| Enzyme Sequence | MSLATRRFGAAAALLVAACVLCTAPAWAQNETTGTGMVKTKSAFRWIRPPPARPPPFRRPPPAQTPYVHKVEYTELQILCPQTIDSVTGYPMDDPRCNVPRATVAAGEEALTIRNEFELLNGDVLNVTLEEVDTPENPSRRRLLSIIREEQRTGRVLLATSAELPTPTFRLKSLKSILKGSQKEIYAGKPIDLRTIVYIMDFSSCKLSGWSAPATLTPEKVTSDMLRGASAPTNNLANYYGACSYEKTLFNPDNFLVLGPVPVPCIGGVTPPPRPPRPPRPPPRAGSTISSLSRRNDTYDDWWDLSKYCTASEQQAWERAAEAYAQAIVAQDPNSATGKKLQGILQWRERRRNIYILPPGVKCSWSGYADVTCTSATCSAYVRGYSDTNAMQVIMHEAMHNYGLEHAGRGTLEYGDATDVMGDFNKAGKGLLCPNAPNMYRIGWAKPINEPGVAPFQNATGAWGNLTAANFTTDPWIRGLVIPAQGTRDDNMIVVNVGAQSTRDGAMKATGAQAYYFSYRIKNTTAGGYDSGLTLDFHKKVLVHAYNGIQSERVFGFKSNLLDWGPNFQSRSNTWTSPFLAYNNGLGGGVRLVVQSTSDTQAVVDICRISENGKELSCDDGIDNDCDGLQDNEDPDCQ |
| Enzyme Length | 638 |
| Uniprot Accession Number | P31178 |
| Absorption | |
| Active Site | ACT_SITE 397; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleavage of the proline- and hydroxyproline-rich proteins of the Chlamydomonas cell wall. Also cleaves azocasein, gelatin and Leu-Trp-Met-|-Arg-Phe-Ala.; EC=3.4.24.38; |
| DNA Binding | |
| EC Number | 3.4.24.38 |
| Enzyme Function | FUNCTION: Mediates digestion of the cell walls of the 2 mating type gametes during mating as a necessary prelude to cell fusion. This enzyme acts specifically on the framework proteins (inner wall) of the cell wall, cleaving several model peptides at specific sites. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Compositional bias (1); Glycosylation (7); Metal binding (4); Motif (1); Propeptide (1); Region (1); Signal peptide (1) |
| Keywords | Cell wall;Cell wall biogenesis/degradation;Direct protein sequencing;Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Periplasm;Protease;Secreted;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | INDUCTION: By the signal of flagellar agglutination between gametes of the opposite mating type. |
| Subcellular Location | SUBCELLULAR LOCATION: Periplasm. Secreted, cell wall. Note=Stored in the periplasm of gametes until its release. Secreted concurrently with release of the cell walls. |
| Modified Residue | |
| Post Translational Modification | PTM: Present in 2 forms: an inactive V-form in vegetative cells and an active and soluble G-form. The V-form enzyme may be converted to the G-form enzyme during gametic differentiation under nitrogen-starved conditions. |
| Signal Peptide | SIGNAL 1..28; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 95..102; /note=Cysteine switch; /evidence=ECO:0000250 |
| Gene Encoded By | |
| Mass | 69,833 |
| Kinetics | |
| Metal Binding | METAL 97; /note=Zinc; in inhibited form; /evidence=ECO:0000250; METAL 396; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 400; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 406; /note=Zinc; catalytic; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.38; |