Detail Information for IndEnz0002018497
IED ID IndEnz0002018497
Enzyme Type ID protease018497
Protein Name Autolysin
EC 3.4.24.38
Gamete lytic enzyme
GLE
Gametolysin
Gene Name
Organism Chlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Chlorophyta core chlorophytes Chlorophyceae CS clade Chlamydomonadales Chlamydomonadaceae Chlamydomonas Chlamydomonas reinhardtii (Chlamydomonas smithii)
Enzyme Sequence MSLATRRFGAAAALLVAACVLCTAPAWAQNETTGTGMVKTKSAFRWIRPPPARPPPFRRPPPAQTPYVHKVEYTELQILCPQTIDSVTGYPMDDPRCNVPRATVAAGEEALTIRNEFELLNGDVLNVTLEEVDTPENPSRRRLLSIIREEQRTGRVLLATSAELPTPTFRLKSLKSILKGSQKEIYAGKPIDLRTIVYIMDFSSCKLSGWSAPATLTPEKVTSDMLRGASAPTNNLANYYGACSYEKTLFNPDNFLVLGPVPVPCIGGVTPPPRPPRPPRPPPRAGSTISSLSRRNDTYDDWWDLSKYCTASEQQAWERAAEAYAQAIVAQDPNSATGKKLQGILQWRERRRNIYILPPGVKCSWSGYADVTCTSATCSAYVRGYSDTNAMQVIMHEAMHNYGLEHAGRGTLEYGDATDVMGDFNKAGKGLLCPNAPNMYRIGWAKPINEPGVAPFQNATGAWGNLTAANFTTDPWIRGLVIPAQGTRDDNMIVVNVGAQSTRDGAMKATGAQAYYFSYRIKNTTAGGYDSGLTLDFHKKVLVHAYNGIQSERVFGFKSNLLDWGPNFQSRSNTWTSPFLAYNNGLGGGVRLVVQSTSDTQAVVDICRISENGKELSCDDGIDNDCDGLQDNEDPDCQ
Enzyme Length 638
Uniprot Accession Number P31178
Absorption
Active Site ACT_SITE 397; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Cleavage of the proline- and hydroxyproline-rich proteins of the Chlamydomonas cell wall. Also cleaves azocasein, gelatin and Leu-Trp-Met-|-Arg-Phe-Ala.; EC=3.4.24.38;
DNA Binding
EC Number 3.4.24.38
Enzyme Function FUNCTION: Mediates digestion of the cell walls of the 2 mating type gametes during mating as a necessary prelude to cell fusion. This enzyme acts specifically on the framework proteins (inner wall) of the cell wall, cleaving several model peptides at specific sites.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Compositional bias (1); Glycosylation (7); Metal binding (4); Motif (1); Propeptide (1); Region (1); Signal peptide (1)
Keywords Cell wall;Cell wall biogenesis/degradation;Direct protein sequencing;Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Periplasm;Protease;Secreted;Signal;Zinc;Zymogen
Interact With
Induction INDUCTION: By the signal of flagellar agglutination between gametes of the opposite mating type.
Subcellular Location SUBCELLULAR LOCATION: Periplasm. Secreted, cell wall. Note=Stored in the periplasm of gametes until its release. Secreted concurrently with release of the cell walls.
Modified Residue
Post Translational Modification PTM: Present in 2 forms: an inactive V-form in vegetative cells and an active and soluble G-form. The V-form enzyme may be converted to the G-form enzyme during gametic differentiation under nitrogen-starved conditions.
Signal Peptide SIGNAL 1..28; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif MOTIF 95..102; /note=Cysteine switch; /evidence=ECO:0000250
Gene Encoded By
Mass 69,833
Kinetics
Metal Binding METAL 97; /note=Zinc; in inhibited form; /evidence=ECO:0000250; METAL 396; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 400; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 406; /note=Zinc; catalytic; /evidence=ECO:0000250
Rhea ID
Cross Reference Brenda 3.4.24.38;