| IED ID | IndEnz0002018519 |
| Enzyme Type ID | protease018519 |
| Protein Name |
Probable aspartic proteinase GIP1 EC 3.4.23.- Glucanase inhibitor protein 1 GmGIP1 |
| Gene Name | GIP1 GLYMA_03G151900 |
| Organism | Glycine max (Soybean) (Glycine hispida) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Fabales Fabaceae Papilionoideae 50 kb inversion clade NPAAA clade indigoferoid/millettioid clade Phaseoleae Glycine Glycine subgen. Soja Glycine max (Soybean) (Glycine hispida) |
| Enzyme Sequence | MPPPLPSLCNFNLAILFLFLTPTFQIPLIAPISKDDTTQLYTLSVFLKTPLQPTKLHLHLGSSLSWVLCDSTYTSSSSHHIPCNTPLCNSFPSNACSNNSSLCALFPENPVTRNTLLDTALIDSLALPTYDASSSLVLISDFIFSCATAHLLQGLAANALGLASLGRSNYSLPAQISTSLTSPRSFTLCLPASSANTGAAIFASTASSFLFSSKIDLTYTQLIVNPVADTVVTDNPQPSDEYFINLTSIKINGKPLYINSSILTVDQTGFGGTKISTAEPYTVLETSIYRLFVQRFVNESSAFNLTVTEAVEPFGVCYPAGDLTETRVGPAVPTVDLVMHSEDVFWRIFGGNSMVRVAKGGVDVWCLGFVDGGTRGRTPIVIGGHQLEDNLMQFDLDSNRFGFTSTLLLQDAKCSNLKVNNFANGIK |
| Enzyme Length | 427 |
| Uniprot Accession Number | I1JNS6 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.23.- |
| Enzyme Function | FUNCTION: Involved in plant defense against Phytophtora sojae (PubMed:28082413). Contributes positively to soybean resistance against P.sojae (PubMed:28082413). Binds the P.sojae xyloglucanase XEG1 and inhibits its cell wall degrading enzyme activity and its contribution as P.sojae virulence factor (PubMed:28082413). XEG1 acts as an important virulence factor during P.sojae infection but also acts as a pathogen-associated molecular pattern (PAMP) in soybean and solanaceous species, where it can trigger defense responses including cell death (PubMed:26163574). {ECO:0000269|PubMed:26163574, ECO:0000269|PubMed:28082413}.; FUNCTION: (Microbial infection) Possesses stronger binding affinity with XLP1, a truncated paralog of P.sojae XEG1 which has no enzyme activity. Is impaired in its inhibitor activity towards the P.sojae xyloglucanase XEG1 when hijacked by XLP1 binding. {ECO:0000269|PubMed:28082413}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Domain (1); Glycosylation (6); Mutagenesis (6); Signal peptide (1) |
| Keywords | Apoplast;Aspartyl protease;Glycoprotein;Hydrolase;Plant defense;Protease;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast {ECO:0000305|PubMed:28082413}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..25; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 45,958 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |