Detail Information for IndEnz0002018559
IED ID IndEnz0002018559
Enzyme Type ID protease018559
Protein Name Lysin
EC 3.5.1.28
Cell wall hydrolase
Lytic amidase
N-acetylmuramoyl-L-alanine amidase
Gene Name PAL
Organism Pneumococcus phage Dp-1 (Bacteriophage Dp-1)
Taxonomic Lineage Viruses Duplodnaviria Heunggongvirae Uroviricota Caudoviricetes Caudovirales Siphoviridae (phages with long non-contractile tails) unclassified Siphoviridae Pneumococcus phage Dp-1 (Bacteriophage Dp-1)
Enzyme Sequence MGVDIEKGVAWMQARKGRVSYSMDFRDGPDSYDCSSSMYYALRSAGASSAGWAVNTEYMHAWLIENGYELISENAPWDAKRGDIFIWGRKGASAGAGGHTGMFIDSDNIIHCNYAYDGISVNDHDERWYYAGQPYYYVYRLTNANAQPAEKKLGWQKDATGFWYARANGTYPKDEFEYIEENKSWFYFDDQGYMLAEKWLKHTDGNWYWFDRDGYMATSWKRIGESWYYFNRDGSMVTGWIKYYDNWYYCDATNGDMKSNAFIRYNDGWYLLLPDGRLADKPQFTVEPDGLITAKV
Enzyme Length 296
Uniprot Accession Number O03979
Absorption
Active Site ACT_SITE 34; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01284; ACT_SITE 99; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01284; ACT_SITE 111; /evidence=ECO:0000255|PROSITE-ProRule:PRU01284
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.; EC=3.5.1.28;
DNA Binding
EC Number 3.5.1.28
Enzyme Function FUNCTION: Lysis of bacterial host cell wall.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Domain (1); Repeat (6)
Keywords Antimicrobial;Bacteriolytic enzyme;Cell wall biogenesis/degradation;Hydrolase;Pharmaceutical;Protease;Repeat;Thiol protease
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Host cell wall. Note=Binds to the choline residues present in the cell wall substrate.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 34,453
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda