| IED ID | IndEnz0002018559 |
| Enzyme Type ID | protease018559 |
| Protein Name |
Lysin EC 3.5.1.28 Cell wall hydrolase Lytic amidase N-acetylmuramoyl-L-alanine amidase |
| Gene Name | PAL |
| Organism | Pneumococcus phage Dp-1 (Bacteriophage Dp-1) |
| Taxonomic Lineage | Viruses Duplodnaviria Heunggongvirae Uroviricota Caudoviricetes Caudovirales Siphoviridae (phages with long non-contractile tails) unclassified Siphoviridae Pneumococcus phage Dp-1 (Bacteriophage Dp-1) |
| Enzyme Sequence | MGVDIEKGVAWMQARKGRVSYSMDFRDGPDSYDCSSSMYYALRSAGASSAGWAVNTEYMHAWLIENGYELISENAPWDAKRGDIFIWGRKGASAGAGGHTGMFIDSDNIIHCNYAYDGISVNDHDERWYYAGQPYYYVYRLTNANAQPAEKKLGWQKDATGFWYARANGTYPKDEFEYIEENKSWFYFDDQGYMLAEKWLKHTDGNWYWFDRDGYMATSWKRIGESWYYFNRDGSMVTGWIKYYDNWYYCDATNGDMKSNAFIRYNDGWYLLLPDGRLADKPQFTVEPDGLITAKV |
| Enzyme Length | 296 |
| Uniprot Accession Number | O03979 |
| Absorption | |
| Active Site | ACT_SITE 34; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01284; ACT_SITE 99; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01284; ACT_SITE 111; /evidence=ECO:0000255|PROSITE-ProRule:PRU01284 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.; EC=3.5.1.28; |
| DNA Binding | |
| EC Number | 3.5.1.28 |
| Enzyme Function | FUNCTION: Lysis of bacterial host cell wall. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (1); Repeat (6) |
| Keywords | Antimicrobial;Bacteriolytic enzyme;Cell wall biogenesis/degradation;Hydrolase;Pharmaceutical;Protease;Repeat;Thiol protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Host cell wall. Note=Binds to the choline residues present in the cell wall substrate. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 34,453 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |