Detail Information for IndEnz0002018560
IED ID IndEnz0002018560
Enzyme Type ID protease018560
Protein Name Protein AMBP
Cleaved into: Alpha-1-microglobulin; Inter-alpha-trypsin inhibitor light chain
ITI-LC
Bikunin
HI-30

Fragment
Gene Name
Organism Pleuronectes platessa (European plaice)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Euteleosteomorpha Neoteleostei Eurypterygia Ctenosquamata Acanthomorphata Euacanthomorphacea Percomorphaceae Carangaria Pleuronectiformes (flatfishes) Pleuronectoidei Pleuronectidae (righteye flounders) Pleuronectes Pleuronectes platessa (European plaice)
Enzyme Sequence RLKTTVVLVPLLLLGWTGTLQGLPVLPEPLYPTQENFDLTRFVGTWHDVALTSSCPHMQRNRADAAIGKLVLEKDTGNKLKVTRTRLRHGTCVEMSGEYELTSTPGRIFYHIDRWDADVDAYVVHTNYDEYAIIIMSKQKTSGENSTSLKLYSRTMSVRDTVLDDFKTLVRHQGMSDDTIIIKQNKGDCIPGEQVEEAPSQPEPKRLRRQVLPTLALSDEEGSGDMSALFNDSEACKAAPETGPCFGFIQGFFYNSTSMRCELFTYGGCLGNQNNFVTVRECLQRCRTEAVCRLPMAPEPCTGQPTIWAFDFVTGSCMPYKDGICQANANQFYSRAECQEYCGVIKDDGELLTAS
Enzyme Length 355
Uniprot Accession Number P36992
Absorption
Active Site
Activity Regulation
Binding Site BINDING 55; /note=3-hydroxy-L-kynurenine; binds multimeric 3-hydroxykynurenine chromophore; covalent; /evidence=ECO:0000250; BINDING 138; /note=3-hydroxy-L-kynurenine; binds multimeric 3-hydroxykynurenine chromophore; covalent; /evidence=ECO:0000250; BINDING 150; /note=3-hydroxy-L-kynurenine; binds multimeric 3-hydroxykynurenine chromophore; covalent; /evidence=ECO:0000250
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Binding site (3); Chain (2); Disulfide bond (7); Domain (2); Glycosylation (3); Non-terminal residue (1); Signal peptide (1)
Keywords Chromophore;Cleavage on pair of basic residues;Disulfide bond;Glycoprotein;Protease inhibitor;Repeat;Secreted;Serine protease inhibitor;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
Modified Residue
Post Translational Modification PTM: The precursor is proteolytically processed into two separately functioning proteins. {ECO:0000250}.; PTM: 3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-55, Lys-138, and Lys-150 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins (By similarity). {ECO:0000250}.
Signal Peptide SIGNAL <1..?; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 39,669
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda