Detail Information for IndEnz0002018580
IED ID IndEnz0002018580
Enzyme Type ID protease018580
Protein Name Probable cytosol aminopeptidase
EC 3.4.11.1
Leucine aminopeptidase
LAP
EC 3.4.11.10
Leucyl aminopeptidase
Gene Name pepA RALTA_A2464
Organism Cupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CCUG 44338 / CIP 107171 / LMG 19424 / R1) (Ralstonia taiwanensis (strain LMG 19424))
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Betaproteobacteria Burkholderiales Burkholderiaceae Cupriavidus Cupriavidus taiwanensis Cupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CCUG 44338 / CIP 107171 / LMG 19424 / R1) (Ralstonia taiwanensis (strain LMG 19424))
Enzyme Sequence MEFSTKALDWSKAGQNGFLATKTDCLVVGLFEGQNLGGVAKALDVATKGLVGRLVKQGDFEGKRGTHLMLHEVAGVGAARVLLVGLGKEADFTDKAFADAVRTAVRALSSTRATSALWCLAQQAPQQRDVSWAVITTITLVREAGYRLLERHPGLKRANAQNAASGKPNGNDKSSLRKVVIAVDSGDARAATQAAVRGTAIANGMELTRDLGNLPSNICTPTYLANTARSIAKRHKLKAEILGRKQIEALNMGAFLAVTKGSAEPPQFIVLRYDGGGARQAPVVLVGKGITFDTGGISLKPGEGMDEMKYDMCGAASVLGTIQAVAEMGLKLNVVAVVPTCENMPSGVATKPGDVVTSMSGQTIEILNTDAEGRLILCDALTYVERFKPAAVIDVATLTGACIIALGHVNSGLYARSDALADALLQAGRRAMDTAWRMPLDDEYQDQLKSNFADMGNIGGRPAGSVTAACFLARFTEKYDWAHLDIAGTAWKSGAAKGATGRPVPLLTQFLMDRAA
Enzyme Length 516
Uniprot Accession Number B3R663
Absorption
Active Site ACT_SITE 300; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; ACT_SITE 374; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00181};
DNA Binding
EC Number 3.4.11.1; 3.4.11.10
Enzyme Function FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. {ECO:0000255|HAMAP-Rule:MF_00181}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (7)
Keywords Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00181}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 54,331
Kinetics
Metal Binding METAL 288; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 293; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 293; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 311; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 370; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 372; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 372; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Rhea ID
Cross Reference Brenda