Detail Information for IndEnz0002018602
IED ID IndEnz0002018602
Enzyme Type ID protease018602
Protein Name Probable cytosol aminopeptidase
EC 3.4.11.1
Leucine aminopeptidase
LAP
EC 3.4.11.10
Leucyl aminopeptidase
Gene Name pepA Nmul_A0746
Organism Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Betaproteobacteria Nitrosomonadales Nitrosomonadaceae Nitrosospira Nitrosospira multiformis Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)
Enzyme Sequence MKFNIKNGTPEKQRSACVVAGIFEQQKLTPAAEALDKSANGYITGILSRGDMKGKAGATLMLHNVPNSACERVLLVGLGKEQELGDKGYRDAVRAVFKALSDTGAADATLFLLEAPVKNRDLSWKVLQIAVGGLESMYRFDRLKSKVEEAEPKLQKITLGIIKSLTEEEQTASEEALQQGLAVGDGMSLAKDLGNLAPNICTPTYLAEQAMNMAKTYKLKVTVLEQKDMEDLGMGALLAVARGSRQPPKLIALEYWGGAKKGKPVVLVGKGVTFDTGGISLKPAGEMDEMKYDMCGAASVLGTVHAVAKMGLPINVVGIIPATENMPGGNATKPGDVVTSMSGQTIEILNTDAEGRLILCDALAYTERYEPEAVIDIATLTGACVIALGHVASGLLSNDDELARELLDASERAVDRAWRLPLFDEYQEQLKSNFADVANIGGRAAGTITAACFLARFTKKYRWAHLDIAGTAWKSGKEKGATGRPVPLLTQFLISRTTRQPGSTGETGSRKNRRKSKE
Enzyme Length 518
Uniprot Accession Number Q2YB18
Absorption
Active Site ACT_SITE 282; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; ACT_SITE 356; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; EC=3.4.11.1; Evidence={ECO:0000255|HAMAP-Rule:MF_00181}; CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.; EC=3.4.11.10; Evidence={ECO:0000255|HAMAP-Rule:MF_00181};
DNA Binding
EC Number 3.4.11.1; 3.4.11.10
Enzyme Function FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. {ECO:0000255|HAMAP-Rule:MF_00181}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (7); Region (1)
Keywords Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease;Reference proteome
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00181}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 55,476
Kinetics
Metal Binding METAL 270; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 275; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 275; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 293; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 352; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 354; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00181; METAL 354; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00181
Rhea ID
Cross Reference Brenda