| IED ID | IndEnz0002018620 |
| Enzyme Type ID | protease018620 |
| Protein Name |
Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13 EC 2.4.1.141 EC 3.4.19.12 Asparagine-linked glycosylation 13 homolog Glycosyltransferase 28 domain-containing protein 1 UDP-N-acetylglucosamine transferase subunit ALG13 homolog |
| Gene Name | ALG13 CXorf45 GLT28D1 MDS031 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MKCVFVTVGTTSFDDLIACVSAPDSLQKIESLGYNRLILQIGRGTVVPEPFSTESFTLDVYRYKDSLKEDIQKADLVISHAGAGSCLETLEKGKPLVVVINEKLMNNHQLELAKQLHKEGHLFYCTCRVLTCPGQAKSIASAPGKCQDSAALTSTAFSGLDFGLLSGYLHKQALVTATHPTCTLLFPSCHAFFPLPLTPTLYKMHKGWKNYCSQKSLNEASMDEYLGSLGLFRKLTAKDASCLFRAISEQLFCSQVHHLEIRKACVSYMRENQQTFESYVEGSFEKYLERLGDPKESAGQLEIRALSLIYNRDFILYRFPGKPPTYVTDNGYEDKILLCYSSSGHYDSVYSKQFQSSAAVCQAVLYEILYKDVFVVDEEELKTAIKLFRSGSKKNRNNAVTGSEDAHTDYKSSNQNRMEEWGACYNAENIPEGYNKGTEETKSPENPSKMPFPYKVLKALDPEIYRNVEFDVWLDSRKELQKSDYMEYAGRQYYLGDKCQVCLESEGRYYNAHIQEVGNENNSVTVFIEELAEKHVVPLANLKPVTQVMSVPAWNAMPSRKGRGYQKMPGGYVPEIVISEMDIKQQKKMFKKIRGKEVYMTMAYGKGDPLLPPRLQHSMHYGHDPPMHYSQTAGNVMSNEHFHPQHPSPRQGRGYGMPRNSSRFINRHNMPGPKVDFYPGPGKRCCQSYDNFSYRSRSFRRSHRQMSCVNKESQYGFTPGNGQMPRGLEETITFYEVEEGDETAYPTLPNHGGPSTMVPATSGYCVGRRGHSSGKQTLNLEEGNGQSENGRYHEEYLYRAEPDYETSGVYSTTASTANLSLQDRKSCSMSPQDTVTSYNYPQKMMGNIAAVAASCANNVPAPVLSNGAAANQAISTTSVSSQNAIQPLFVSPPTHGRPVIASPSYPCHSAIPHAGASLPPPPPPPPPPPPPPPPPPPPPPPPPPPALDVGETSNLQPPPPLPPPPYSCDPSGSDLPQDTKVLQYYFNLGLQCYYHSYWHSMVYVPQMQQQLHVENYPVYTEPPLVDQTVPQCYSEVRREDGIQAEASANDTFPNADSSSVPHGAVYYPVMSDPYGQPPLPGFDSCLPVVPDYSCVPPWHPVGTAYGGSSQIHGAINPGPIGCIAPSPPASHYVPQGM |
| Enzyme Length | 1137 |
| Uniprot Accession Number | Q9NP73 |
| Absorption | |
| Active Site | ACT_SITE 239; /note=For deubiquitinase activity; /evidence=ECO:0000250; ACT_SITE 242; /note=Nucleophile; for deubiquitinase activity; /evidence=ECO:0000250; ACT_SITE 345; /note=For deubiquitinase activity; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=N-acetyl-alpha-D-glucosaminyl-diphosphodolichol + UDP-N-acetyl-alpha-D-glucosamine = H(+) + N,N'-diacetylchitobiosyl diphosphodolichol + UDP; Xref=Rhea:RHEA:23380, Rhea:RHEA-COMP:9519, Rhea:RHEA-COMP:9520, ChEBI:CHEBI:15378, ChEBI:CHEBI:57269, ChEBI:CHEBI:57705, ChEBI:CHEBI:58223, ChEBI:CHEBI:58427; EC=2.4.1.141; CATALYTIC ACTIVITY: Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| DNA Binding | |
| EC Number | 2.4.1.141; 3.4.19.12 |
| Enzyme Function | FUNCTION: [Isoform 1]: Possible multifunctional enzyme with both glycosyltransferase and deubiquitinase activities.; FUNCTION: [Isoform 2]: May be involved in protein N-glycosylation, second step of the dolichol-linked oligosaccharide pathway. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (6); Chain (1); Compositional bias (2); Domain (2); Erroneous initiation (5); Erroneous termination (1); Natural variant (2); Region (4); Sequence conflict (11) |
| Keywords | Alternative splicing;Congenital disorder of glycosylation;Disease variant;Endoplasmic reticulum;Epilepsy;Glycosyltransferase;Hydrolase;Multifunctional enzyme;Protease;Reference proteome;Thiol protease;Transferase;Ubl conjugation pathway |
| Interact With | O95429; P49639; O60336; Q01974 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Isoform 2]: Endoplasmic reticulum {ECO:0000305}. Note=Could be recruited to the cytosolic face of the endoplasmic reticulum membrane through its interaction with ALG14. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 16713569; 18985028; 20711500; 23404334; 24255178; |
| Motif | |
| Gene Encoded By | |
| Mass | 126,056 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:23380 |
| Cross Reference Brenda |