| IED ID | IndEnz0002018622 |
| Enzyme Type ID | protease018622 |
| Protein Name |
Calpain small subunit 1 CSS1 Calcium-activated neutral proteinase small subunit CANP small subunit Calcium-dependent protease small subunit CDPS Calcium-dependent protease small subunit 1 Calpain regulatory subunit |
| Gene Name | Capns1 Capn4 Css1 |
| Organism | Rattus norvegicus (Rat) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat) |
| Enzyme Sequence | MFLVNSFLKGGGGGGGGGGLGGGLGNVLGGLISGAAGGGGGGGGGGGMGLGGGGGGGGTAMRILGGVISAISEAAAQYNPEPPPPRSHYSNIEANESEEERQFRKLFVQLAGDDMEVSATELMNILNKVVTRHPDLKTDGFGIDTCRSMVAVMDSDTTGKLGFEEFKYLWNNIKKWQGIYKRFDTDRSGTIGSNELPGAFEAAGFHLNQHIYSMIIRRYSDETGNMDFDNFISCLVRLDAMFRAFRSLDKNGTGQIQVNIQEWLQLTMYS |
| Enzyme Length | 270 |
| Uniprot Accession Number | Q64537 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | CA_BIND 110..121; /note="1"; /evidence="ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0"; CA_BIND 154..165; /note="2"; /evidence="ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0"; CA_BIND 184..195; /note="3"; /evidence="ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0" |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Essential for embryonic development (By similarity). {ECO:0000250|UniProtKB:O88456}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (4); Calcium binding (3); Chain (1); Domain (5); Helix (9); Metal binding (16); Modified residue (3); Sequence conflict (1); Turn (3) |
| Keywords | 3D-structure;Acetylation;Calcium;Cell membrane;Cytoplasm;Direct protein sequencing;Membrane;Metal-binding;Phosphoprotein;Reference proteome;Repeat |
| Interact With | Q07009 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon calcium binding (By similarity). Allows the formation of the homodimer and also appears to mediate the contact between the large catalytic subunit and small regulatory subunit for the formation of the heterodimer. {ECO:0000250}. |
| Modified Residue | MOD_RES 1; /note=N-acetylmethionine; /evidence=ECO:0000250|UniProtKB:P04632; MOD_RES 6; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P04632; MOD_RES 181; /note=N6-acetyllysine; /evidence=ECO:0000250|UniProtKB:P04632 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (8) |
| Cross Reference PDB | 1AJ5; 1DF0; 1DVI; 1NP8; 1QXP; 1U5I; 3BOW; 3DF0; |
| Mapped Pubmed ID | 10601010; 10639123; 11893336; 12665854; 12684003; 14656436; 15491615; 16396496; 18559257; |
| Motif | |
| Gene Encoded By | |
| Mass | 28,570 |
| Kinetics | |
| Metal Binding | METAL 111; /note="Calcium 1; via carbonyl oxygen"; /evidence="ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0"; METAL 114; /note="Calcium 1"; /evidence="ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0"; METAL 116; /note="Calcium 1; via carbonyl oxygen"; /evidence="ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0"; METAL 121; /note="Calcium 1"; /evidence="ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0"; METAL 139; /note="Calcium 4"; /evidence="ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0"; METAL 154; /note="Calcium 2"; /evidence="ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0"; METAL 156; /note="Calcium 2"; /evidence="ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0"; METAL 158; /note="Calcium 2; via carbonyl oxygen"; /evidence="ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0"; METAL 160; /note="Calcium 2; via carbonyl oxygen"; /evidence="ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0"; METAL 165; /note="Calcium 2"; /evidence="ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0"; METAL 184; /note="Calcium 3"; /evidence="ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0"; METAL 186; /note="Calcium 3"; /evidence="ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0"; METAL 188; /note="Calcium 3; via carbonyl oxygen"; /evidence="ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0"; METAL 190; /note="Calcium 3; via carbonyl oxygen"; /evidence="ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0"; METAL 195; /note="Calcium 3"; /evidence="ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0"; METAL 227; /note="Calcium 4"; /evidence="ECO:0000269|PubMed:19020622, ECO:0000269|PubMed:19020623, ECO:0000269|PubMed:9228945, ECO:0007744|PDB:1DVI, ECO:0007744|PDB:3BOW, ECO:0007744|PDB:3DF0" |
| Rhea ID | |
| Cross Reference Brenda | 3.4.22.B24; |