Detail Information for IndEnz0002018629
IED ID IndEnz0002018629
Enzyme Type ID protease018629
Protein Name C5a peptidase
EC 3.4.21.110
SCP
Gene Name scpA SPs1724
Organism Streptococcus pyogenes serotype M3 (strain SSI-1)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus Streptococcus pyogenes Streptococcus pyogenes serotype M3 Streptococcus pyogenes serotype M3 (strain SSI-1)
Enzyme Sequence MRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPATEQAVEVPQQTAVSEEAPSSSSKETNPPQTPDDAEETVADKANDLAPQAPAKTADIPATSKETIRDLNDPSHVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKSKARYQSKEDLEKAKKDHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETKEPYRLEGAMPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDETKKAFDYAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSPDKQLTETATVKTADQQDKEMPVLSTNRFEPNKAYDYAYANRGTKEDDFKDVKGKIALIERGDIDFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRKDGLLLKDNSKKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAKLSGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYFSPRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELYYQATVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMKNGYFLEGFVRFKQDPTKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSDAKDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGTFAKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGDVVWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPISSGAKEQHTDFDVIVDNTTPEAATSATFSAEDRRLTLASKPKTSQPVYRERIAYTYMDEDLPTTEYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTNLLEGHSNKPEQDGSDQVPDKTPETKPEQDGSGQAPDKKPEAKPEQDGSGQAPDKKPETKPEKDSSGQTPGKTPQKGQPSRTLEKRSSKRALATKASARDQLPTTNDKDTNRLHLLKLVMTTFFFGLVAHIFKTKRQKETKK
Enzyme Length 1169
Uniprot Accession Number P0DD35
Absorption
Active Site ACT_SITE 132; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 195; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 514; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=The primary cleavage site is at 67-His-|-Lys-68 in human C5a with a minor secondary cleavage site at 58-Ala-|-Ser-59.; EC=3.4.21.110;
DNA Binding
EC Number 3.4.21.110
Enzyme Function FUNCTION: This virulence factor of S.pyogenes specifically cleaves the human serum chemotaxin C5a at '68-Lys-|-Asp-69' bond near its C-terminus, destroying its ability to serve as a chemoattractant.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Compositional bias (3); Domain (1); Modified residue (1); Motif (1); Propeptide (1); Region (3); Repeat (4); Signal peptide (1)
Keywords Cell wall;Hydrolase;Peptidoglycan-anchor;Protease;Repeat;Secreted;Serine protease;Signal;Virulence
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000255|PROSITE-ProRule:PRU00477}; Peptidoglycan-anchor {ECO:0000255|PROSITE-ProRule:PRU00477}.
Modified Residue MOD_RES 1132; /note=Pentaglycyl murein peptidoglycan amidated threonine; /evidence=ECO:0000255|PROSITE-ProRule:PRU00477
Post Translational Modification
Signal Peptide SIGNAL 1..31; /evidence=ECO:0000250
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif MOTIF 1129..1133; /note=LPXTG sorting signal; /evidence=ECO:0000255|PROSITE-ProRule:PRU00477
Gene Encoded By
Mass 128,228
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda