IED ID | IndEnz0002018631 |
Enzyme Type ID | protease018631 |
Protein Name |
D-alanyl-D-alanine carboxypeptidase DacA CPase DD-carboxypeptidase DD-peptidase EC 3.4.16.4 Penicillin-binding protein 5 PBP-5 |
Gene Name | dacA |
Organism | Geobacillus stearothermophilus (Bacillus stearothermophilus) |
Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Geobacillus Geobacillus stearothermophilus (Bacillus stearothermophilus) |
Enzyme Sequence | MRRRKQNWLFWLLSICLCLTFGPFQQTVKAESAPLDIRADAAILVDAQTGRILYEKNIDTVLGIASMTKMMTEYLLLDAIKAKRVKWDQMYTPSDYVYRLSQDRALSNVPLRKDGKYTVRELYEAMAIYSANGATVAIAEIIAGSEKNFVKMMNDKAKELGLKDYKFVNATGLSNKDLKGFHPEGTSTNEENVMSARAMAMLAYRLLKDHPEVLKTASIPHKVFREGTKDEIKMDNWNWMLPGLVYGYEGVDGLKTGYTEFAGNCFTGTAKRNGVRLISVVMNAKDASGKTTKEARFKETEKLFNYGFNQYSLETLYPKGYQLKGKETLPVVKGKEKEVRVATGKNLDLLVKNGEEKQYKPVYVLDKKKMTKEGKLVAPLKKGETVGYMTLEYKGDDSLAFLSPDMQKNIRVPLVTTAEVEKANWFVLSMRAVGGLFVDLWTSVAKTVKGWL |
Enzyme Length | 452 |
Uniprot Accession Number | Q05523 |
Absorption | |
Active Site | ACT_SITE 66; /note=Acyl-ester intermediate; /evidence=ECO:0000269|PubMed:111240; ACT_SITE 69; /note=Proton acceptor; /evidence=ECO:0000250; ACT_SITE 130; /evidence=ECO:0000250 |
Activity Regulation | |
Binding Site | BINDING 255; /note=Substrate; /evidence=ECO:0000250 |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.; EC=3.4.16.4; |
DNA Binding | |
EC Number | 3.4.16.4 |
Enzyme Function | FUNCTION: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. |
Temperature Dependency | |
PH Dependency | |
Pathway | PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis. |
nucleotide Binding | |
Features | Active site (3); Binding site (1); Chain (1); Signal peptide (1) |
Keywords | Carboxypeptidase;Cell shape;Cell wall;Cell wall biogenesis/degradation;Direct protein sequencing;Hydrolase;Peptidoglycan synthesis;Protease;Secreted;Signal;Sporulation |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Secreted, cell wall. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..30 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 51,002 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |