Detail Information for IndEnz0002018646
IED ID IndEnz0002018646
Enzyme Type ID protease018646
Protein Name Transcriptional regulatory protein DegU
Protease production enhancer protein
Gene Name degU iep BSU35490
Organism Bacillus subtilis (strain 168)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis (strain 168)
Enzyme Sequence MTKVNIVIIDDHQLFREGVKRILDFEPTFEVVAEGDDGDEAARIVEHYHPDVVIMDINMPNVNGVEATKQLVELYPESKVIILSIHDDENYVTHALKTGARGYLLKEMDADTLIEAVKVVAEGGSYLHPKVTHNLVNEFRRLATSGVSAHPQHEVYPEIRRPLHILTRRECEVLQMLADGKSNRGIGESLFISEKTVKNHVSNILQKMNVNDRTQAVVVAIKNGWVEMR
Enzyme Length 229
Uniprot Accession Number P13800
Absorption
Active Site
Activity Regulation ACTIVITY REGULATION: Phosphorylated and unphosphorylated forms are both active and have different functions. A low concentration of phospho-DegU is sufficient to activate transcription of flagellar genes, but a higher concentration of phospho-DegU is required for transcription of other genes. Phosphorylated DegU is stabilized by DegR. {ECO:0000269|PubMed:1321152, ECO:0000269|PubMed:1459944, ECO:0000269|PubMed:17850253}.
Binding Site
Calcium Binding
catalytic Activity
DNA Binding DNA_BIND 183..202; /note=H-T-H motif; /evidence=ECO:0000255|PROSITE-ProRule:PRU00411
EC Number
Enzyme Function FUNCTION: Member of the two-component regulatory system DegS/DegU, which plays an important role in the transition growth phase. Involved in the control of expression of different cellular functions, including production of degradative enzymes such as the neutral and alkaline proteases, flagellum formation, biofilm formation, and competence for DNA uptake. Positively or negatively regulates expression of many different genes. The phosphorylated form is required for synthesis of degradative enzymes, flagellum formation and biofilm formation. The unphosphorylated form is required for expression of genetic competence, via induction of comK. {ECO:0000269|PubMed:12471443, ECO:0000269|PubMed:1321152, ECO:0000269|PubMed:17850253, ECO:0000269|PubMed:1901568, ECO:0000269|PubMed:2123196, ECO:0000269|PubMed:8955341}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); DNA binding (1); Domain (2); Modified residue (1); Mutagenesis (6)
Keywords Activator;Cytoplasm;DNA-binding;Phosphoprotein;Reference proteome;Repressor;Transcription;Transcription regulation;Two-component regulatory system
Interact With
Induction INDUCTION: Phosphorylated DegU activates its own expression. {ECO:0000269|PubMed:17850253}.
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
Modified Residue MOD_RES 56; /note=4-aspartylphosphate; /evidence=ECO:0000305
Post Translational Modification PTM: Phosphorylated and dephosphorylated by DegS. {ECO:0000269|PubMed:1901568, ECO:0000269|PubMed:2123196}.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 25,867
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda