| IED ID | IndEnz0002018654 |
| Enzyme Type ID | protease018654 |
| Protein Name |
ATP-dependent zinc metalloprotease FTSH 12, chloroplastic AtFTSH12 EC 3.4.24.- |
| Gene Name | FTSH12 At1g79560 T8K14.2 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MEIAISYKPNPLISSSTQLLKRSKSFGLVRFPAKYGLGATRKKQLFRVYASESSSGSSSNSDGGFSWVRLAQSIRLGAERIGEKIGESVKTEIGFDSEEASGRVNEYVARVKDSVHKGHHELTRFKNETVPSFIDWNKWEHWKDIRNWDGKRVAALFIYAFALLLSCQRVYVAIQAPRVERERRELTESFMEALIPEPSPGNIEKFKRNMWRKATPKGLKLKRFIEAPDGTLVHDSSYVGENAWDDDLETTEGSLKKIIGRNARIQTEAKKKLSQDLGVSGEIGDSVGNWRERLATWKEMLEREKLSEQLNSSAAKYVVEFDMKEVEKSLREDVIGRTSETEGTRALWISKRWWRYRPKLPYTYFLQKLDSSEVAAVVFTEDLKRLYVTMKEGFPLEYIVDIPLDPYLFETICNAGVEVDLLQKRQIHYFMKVFIALLPGILILWFIRESAMLLLITSKRFLYKKYNQLFDMAYAENFILPVGDVSETKSMYKEVVLGGDVWDLLDELMIYMGNPMQYYEKDVAFVRGVLLSGPPGTGKTLFARTLAKESGLPFVFASGAEFTDSEKSGAAKINEMFSIARRNAPAFVFVDEIDAIAGRHARKDPRRRATFEALIAQLDGEKEKTGIDRFSLRQAVIFICATNRPDELDLEFVRSGRIDRRLYIGLPDAKQRVQIFGVHSAGKNLAEDIDFGKLVFRTVGFSGADIRNLVNEAAIMSVRKGRSYIYQQDIVDVLDKQLLEGMGVLLTEEEQQKCEQSVSYEKKRLLAVHEAGHIVLAHLFPRFDWHAFSQLLPGGKETAVSVFYPREDMVDQGYTTFGYMKMQMVVAHGGRCAERVVFGDNVTDGGKDDLEKITKIAREMVISPQSARLGLTQLVKKIGMVDLPDNPDGELIKYRWDHPHVMPAEMSVEVSELFTRELTRYIEETEELAMNALRANRHILDLITRELLEKSRITGLEVEEKMKDLSPLMFEDFVKPFQINPDDEELLPHKDRVSYQPVDLRAAPLHRS |
| Enzyme Length | 1008 |
| Uniprot Accession Number | Q9SAJ3 |
| Absorption | |
| Active Site | ACT_SITE 770; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Probable ATP-dependent zinc metallopeptidase. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 533..540; /note=ATP; /evidence=ECO:0000255 |
| Features | Active site (1); Chain (1); Erroneous gene model prediction (1); Metal binding (3); Nucleotide binding (1); Transit peptide (2); Transmembrane (2) |
| Keywords | ATP-binding;Chloroplast;Hydrolase;Membrane;Metal-binding;Metalloprotease;Nucleotide-binding;Plastid;Protease;Reference proteome;Thylakoid;Transit peptide;Transmembrane;Transmembrane helix;Zinc |
| Interact With | |
| Induction | INDUCTION: By high light. {ECO:0000269|PubMed:15266057}. |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane {ECO:0000269|PubMed:14630971}; Multi-pass membrane protein {ECO:0000269|PubMed:14630971}; Stromal side {ECO:0000269|PubMed:14630971}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14576160; 15047901; 15266054; 16618929; 17181860; 18431481; 19812694; 21139083; 22121866; 26909098; 28213560; 29934433; 30309901; 30721974; 31334862; 33216923; 9501126; |
| Motif | |
| Gene Encoded By | |
| Mass | 115,105 |
| Kinetics | |
| Metal Binding | METAL 769; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 773; /note=Zinc; catalytic; /evidence=ECO:0000250; METAL 849; /note=Zinc; catalytic; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.B20; |