| IED ID | IndEnz0002018662 |
| Enzyme Type ID | protease018662 |
| Protein Name |
ATP-dependent zinc metalloprotease FtsH EC 3.4.24.- |
| Gene Name | ftsH aq_936 |
| Organism | Aquifex aeolicus (strain VF5) |
| Taxonomic Lineage | cellular organisms Bacteria Aquificae Aquificae Aquificales Aquificaceae Aquifex Aquifex aeolicus Aquifex aeolicus (strain VF5) |
| Enzyme Sequence | MNALKNFFIWAIIIGAAIVAFNLFEGKREFTTKVSLNEVVKLVEEGKVSYAEVRGNTAIIQTKDGQKLEVTLPPNTNLVDKMVEKGVRVEVANPEPPGGWLVNVFLSWLPILFFIGIWIFLLRQMSGGGNVNRAFNFGKSRAKVYIEEKPKVTFKDVAGIEEVKEEVKEIIEYLKDPVKFQKLGGRPPKGVLLYGEPGVGKTLLAKAIAGEAHVPFISVSGSDFVEMFVGVGAARVRDLFETAKKHAPCIIFIDEIDAVGRARGAIPVGGGHDEREQTLNQLLVEMDGFDTSDGIIVIAATNRPDILDPALLRPGRFDRQIFIPKPDVRGRYEILKVHARNKKLAKDVDLEFVARATPGFTGADLENLLNEAALLAARKGKEEITMEEIEEALDRITMGLERKGMTISPKEKEKIAIHEAGHALMGLVSDDDDKVHKISIIPRGMALGVTQQLPIEDKHIYDKKDLYNKILVLLGGRAAEEVFFGKDGITTGAENDLQRATDLAYRMVSMWGMSDKVGPIAIRRVANPFLGGMTTAVDTSPDLLREIDEEVKRIITEQYEKAKAIVEEYKEPLKAVVKKLLEKETITCEEFVEVFKLYGIELKDKCKKEELFDKDRKSEENKELKSEEVKEEVV |
| Enzyme Length | 634 |
| Uniprot Accession Number | O67077 |
| Absorption | |
| Active Site | ACT_SITE 419; /evidence=ECO:0000255|HAMAP-Rule:MF_01458 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins. {ECO:0000255|HAMAP-Rule:MF_01458}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 195..202; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_01458 |
| Features | Active site (1); Beta strand (13); Chain (1); Helix (22); Metal binding (3); Nucleotide binding (1); Region (1); Topological domain (3); Transmembrane (2); Turn (2) |
| Keywords | 3D-structure;ATP-binding;Cell inner membrane;Cell membrane;Hydrolase;Membrane;Metal-binding;Metalloprotease;Nucleotide-binding;Protease;Reference proteome;Transmembrane;Transmembrane helix;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein; Cytoplasmic side. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (5) |
| Cross Reference PDB | 2DI4; 4WW0; 4Z8X; 6GCN; 6GCO; |
| Mapped Pubmed ID | 12377127; 26057670; 30118817; |
| Motif | |
| Gene Encoded By | |
| Mass | 70,686 |
| Kinetics | |
| Metal Binding | METAL 418; /note=Zinc; catalytic; /evidence=ECO:0000305; METAL 422; /note=Zinc; catalytic; /evidence=ECO:0000305; METAL 496; /note=Zinc; catalytic; /evidence=ECO:0000305 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.B17;3.4.24.B20; |