Detail Information for IndEnz0002018672
IED ID IndEnz0002018672
Enzyme Type ID protease018672
Protein Name Gag polyprotein
Cleaved into: Matrix protein p10; Phosphorylated protein pp21; Protein p3; Protein p8; Protein n; Capsid protein p27; Nucleocapsid protein p14
Gene Name gag
Organism Mouse mammary tumor virus (strain BR6) (MMTV)
Taxonomic Lineage Viruses Riboviria Pararnavirae Artverviricota Revtraviricetes Ortervirales Retroviridae Orthoretrovirinae Betaretrovirus Mouse mammary tumor virus (MMTV) Mouse mammary tumor virus (strain BR6) (MMTV)
Enzyme Sequence MGVSGSKGQKLFVSVLQRLLSERGLHVKESSAIEFYQFLIKVSPWFPEEGGLNLQDWKRVGREMKRYAAEHGTDSIPKQAYPIWLQLREILTEQSDLVLLSAEAKSVTEEELEEGLTGLLSTSSQEKTYGTRGTAYAEIDTEVDKLSEHIYDEPYEEKEKADKNEEKDHVRKIKKVVQRKENSEGKRKEKDSKAFLATDWNDDDLSPEDWDDLEEQAAHYHDDDELILPVKRKVVKKKPQALRRKPLPPVGFAGAMAEAREKGDLTFTFPVVFMGESDEDDTPVWEPLPLKTLKELQSAVRTMGPSAPYTLQVVDMVASQWLTPSDWHQTARATLSPGDYVLWRTEYEEKSKEMVQKAAGKRKGKVSLDMLLGTGQFLSPSSQIKLSKDVLKDVTTNAVLAWRAIPPPGVKKTVLAGLKQGNEESYETFISRLEEAVYRMMPRGEGSDILIKQLAWENANSLCQDLIRPIRKTGTIQDYIRACLDASPAVVQGMAYAAAMRGQKYSTFVKQTYGGGKGGQGAEGPVCFSCGKTGHIRKDCKDEKGSKRAPPGLCPRCKKGYHWKSECKSKFDKDGNPLPPLETNAENSKNL
Enzyme Length 591
Uniprot Accession Number P10258
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: [Matrix protein p10]: Matrix protein. {ECO:0000305}.; FUNCTION: [Nucleocapsid protein p14]: Binds strongly to viral nucleic acids and promotes their aggregation. Also destabilizes the nucleic acids duplexes via highly structured zinc-binding motifs. {ECO:0000305}.; FUNCTION: [Capsid protein p27]: Capsid protein. {ECO:0000305}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (8); Helix (6); Initiator methionine (1); Lipidation (1); Motif (1); Region (2); Site (6); Turn (2); Zinc finger (2)
Keywords 3D-structure;Capsid protein;DNA-binding;Host-virus interaction;Lipoprotein;Metal-binding;Myristate;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;Ribosomal frameshifting;Viral budding;Viral budding via the host ESCRT complexes;Viral matrix protein;Viral nucleoprotein;Viral release from host cell;Virion;Zinc;Zinc-finger
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: [Matrix protein p10]: Virion {ECO:0000269|PubMed:205999}.; SUBCELLULAR LOCATION: [Capsid protein p27]: Virion {ECO:0000269|PubMed:205999}.; SUBCELLULAR LOCATION: [Nucleocapsid protein p14]: Virion {ECO:0000269|PubMed:205999}.
Modified Residue
Post Translational Modification PTM: [Gag polyprotein]: Specific enzymatic cleavages in vivo yield mature proteins. {ECO:0000269|PubMed:1331110}.; PTM: [Gag polyprotein]: Myristoylated. Myristoylation of the matrix (MA) domain mediates the transport and binding of Gag polyproteins to the host plasma membrane and is required for the assembly of viral particles. {ECO:0000269|PubMed:26728401}.
Signal Peptide
Structure 3D X-ray crystallography (3)
Cross Reference PDB 4ZV5; 5HYB; 5I27;
Mapped Pubmed ID 24056256;
Motif MOTIF 305..308; /note=PTAP/PSAP motif; /evidence=ECO:0000305
Gene Encoded By
Mass 66,269
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda