Detail Information for IndEnz0002018675
IED ID IndEnz0002018675
Enzyme Type ID protease018675
Protein Name Gag polyprotein
Cleaved into: Matrix protein p10; Phosphorylated protein pp21; Protein p3; Protein p8; Protein n; Capsid protein p27; Nucleocapsid protein p14
Gene Name gag
Organism Mouse mammary tumor virus (strain C3H) (MMTV)
Taxonomic Lineage Viruses Riboviria Pararnavirae Artverviricota Revtraviricetes Ortervirales Retroviridae Orthoretrovirinae Betaretrovirus Mouse mammary tumor virus (MMTV) Mouse mammary tumor virus (strain C3H) (MMTV)
Enzyme Sequence MGVSGSKGQKLFVSVLQRLLSERGLHVKESSAIEFYQFLIKVSPWFPEEGGLNLQDWKRVGREMKKYAAEHGTDSIPKQAYPIWLQLREILTEQSDLVLLSAEAKSVTEEELEEGLTGLLSASSQEKTYGTRGTAYAEIDTEVDKLSEHIYDEPYEEKEKADKNEEKDHVRKVKKIVQRKENSEHKRKEKDQKAFLATDWNNDDLSPEDWDDLEEQAAHYHDDDELILPVKRKVDKKKPLALRRKPLPPVGFAGAMAEAREKGDLTFTFPVVFMGESDDDDTPVWEPLPLKTLKELQSAVRTMGPSAPYTLQVVDMVASQWLTPSDWHQTARATLSPGDYVLWRTEYEEKSKETVQKTAGKRKGKVSLDMLLGTGQFLSPSSQIKLSKDVLKDVTTNAVLAWRAIPPPGVKKTVLAGLKQGNEESYETFISRLEEAVYRVMPRGEGSDILIKQLAWENANSLCQDLIRPMRKTGTMQDYIRACLDASPAVVQGMAYAAAMRGQKYSTFVKQTYGGGKGGQGSKGPVCFSCGKTGHIKRDCKEEKGSKRAPPGLCPRCKKGYHWKSECKSKFDKDGNPLPPLETNAENSKNL
Enzyme Length 591
Uniprot Accession Number P11284
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: [Matrix protein p10]: Matrix protein. {ECO:0000305}.; FUNCTION: [Nucleocapsid protein p14]: Binds strongly to viral nucleic acids and promote their aggregation. Also destabilizes the nucleic acids duplexes via highly structured zinc-binding motifs. {ECO:0000305}.; FUNCTION: [Capsid protein p27]: Capsid protein. {ECO:0000305}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Beta strand (3); Chain (8); Helix (1); Initiator methionine (1); Lipidation (1); Motif (1); Natural variant (1); Region (2); Sequence conflict (3); Site (6); Turn (4); Zinc finger (2)
Keywords 3D-structure;Capsid protein;DNA-binding;Direct protein sequencing;Host-virus interaction;Lipoprotein;Metal-binding;Myristate;Nucleotide-binding;Phosphoprotein;Reference proteome;Repeat;Ribosomal frameshifting;Viral budding;Viral budding via the host ESCRT complexes;Viral matrix protein;Viral nucleoprotein;Viral release from host cell;Virion;Zinc;Zinc-finger
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: [Matrix protein p10]: Virion {ECO:0000250|UniProtKB:P10258}.; SUBCELLULAR LOCATION: [Capsid protein p27]: Virion {ECO:0000250|UniProtKB:P10258}.; SUBCELLULAR LOCATION: [Nucleocapsid protein p14]: Virion {ECO:0000250|UniProtKB:P10258}.
Modified Residue
Post Translational Modification PTM: [Gag polyprotein]: Myristoylated. Myristoylation of the matrix (MA) domain mediates the transport and binding of Gag polyproteins to the host plasma membrane and is required for the assembly of viral particles. {ECO:0000269|PubMed:2542570}.; PTM: [Gag polyprotein]: Specific enzymatic cleavages in vivo yield mature proteins. {ECO:0000269|PubMed:2542570}.
Signal Peptide
Structure 3D NMR spectroscopy (2)
Cross Reference PDB 1DSQ; 1DSV;
Mapped Pubmed ID -
Motif MOTIF 305..308; /note=PTAP/PSAP motif; /evidence=ECO:0000305
Gene Encoded By
Mass 66,351
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda