Detail Information for IndEnz0002018690
IED ID IndEnz0002018690
Enzyme Type ID protease018690
Protein Name Dipeptidase 3
EC 3.4.13.19
Gene Name Dpep3
Organism Rattus norvegicus (Rat)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Rattus Rattus norvegicus (Rat)
Enzyme Sequence MQPTGPEGPRALSLRPLGHRLSLLGVLLIIPSLWVTCNQTTPSLSSAPTSPGASSAMTTPGIPNDTTTSGVTSDPRLREQALALMRDFPLVDGHNDLPLLLRELFQNKLQDVNLHNFTRGQTSLDRLRDGLVGAQFWSAYIPCQTQDRDAVRVALEQIDLIRRMCSAYPELELVTSADGLNSTQKLACLIGLEGGHSLDTSLAVLRSFYELGVRYLTLTFTCSTPWAESATKFRHHFYTNISGLTSFGEKVVEEMNRIGMMIDLSHASDTLVKQTLEASRAPVIFSHSAARSVCDNLLNVPDDILQLLKKNGGIVMVTLSMGVLQCSLLANVSTVADHFDHIRTVIGSEFIGIGGSYDGSGRFPQGLEDVSTYPVLLEELLRRGWGEQELQGVLRGNLLRVFRQVEQVREKSLGQSPVEVVFPERQQSSTCHSHLLPQSQDAHLKVTKLPSSQVLQRASKAPPCPLLGLVAAVTSPAFTLWLCCSGHR
Enzyme Length 488
Uniprot Accession Number Q5U2X4
Absorption
Active Site
Activity Regulation ACTIVITY REGULATION: Inhibited by L-penicillamine. {ECO:0000250|UniProtKB:Q9DA79}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=an L-aminoacyl-L-amino acid + H2O = 2 an L-alpha-amino acid; Xref=Rhea:RHEA:48940, ChEBI:CHEBI:15377, ChEBI:CHEBI:59869, ChEBI:CHEBI:77460; EC=3.4.13.19; Evidence={ECO:0000250|UniProtKB:Q9DA79, ECO:0000255|PROSITE-ProRule:PRU10073}; CATALYTIC ACTIVITY: Reaction=2 H2O + L-cystine-bis-glycine = 2 glycine + L-cystine; Xref=Rhea:RHEA:60520, ChEBI:CHEBI:15377, ChEBI:CHEBI:35491, ChEBI:CHEBI:57305, ChEBI:CHEBI:143812; Evidence={ECO:0000250|UniProtKB:Q9DA79};
DNA Binding
EC Number 3.4.13.19
Enzyme Function FUNCTION: Dipeptidase that hydrolyzes cystinyl-bis-glycine. Does not hydrolyze leukotriene D4 (LTD4) into leukotriene E4 (LTE4). {ECO:0000250|UniProtKB:Q9DA79}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Disulfide bond (3); Glycosylation (1); Lipidation (1); Metal binding (6); Propeptide (1); Region (1); Signal peptide (1)
Keywords Dipeptidase;Disulfide bond;GPI-anchor;Glycoprotein;Hydrolase;Lipoprotein;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Signal;Zinc
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:Q9DA79}; Lipid-anchor, GPI-anchor {ECO:0000250|UniProtKB:Q9DA79}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..35; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 53,370
Kinetics
Metal Binding METAL 94; /note=Zinc 1; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10073; METAL 96; /note=Zinc 1; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10073; METAL 193; /note=Zinc 1; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10073; METAL 193; /note=Zinc 2; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10073; METAL 266; /note=Zinc 2; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10073; METAL 287; /note=Zinc 2; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10073
Rhea ID RHEA:48940; RHEA:60520
Cross Reference Brenda