Detail Information for IndEnz0002018701
IED ID IndEnz0002018701
Enzyme Type ID protease018701
Protein Name D-aminopeptidase
EC 3.4.11.-
Gene Name dppA dciAA BSU12920
Organism Bacillus subtilis (strain 168)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis (strain 168)
Enzyme Sequence MKLYMSVDMEGISGLPDDTFVDSGKRNYERGRLIMTEEANYCIAEAFNSGCTEVLVNDSHSKMNNLMVEKLHPEADLISGDVKPFSMVEGLDDTFRGALFLGYHARASTPGVMSHSMIFGVRHFYINDRPVGELGLNAYVAGYYDVPVLMVAGDDRAAKEAEELIPNVTTAAVKQTISRSAVKCLSPAKAGRLLTEKTAFALQNKDKVKPLTPPDRPVLSIEFANYGQAEWANLMPGTEIKTGTTTVQFQAKDMLEAYQAMLVMTELAMRTSFC
Enzyme Length 274
Uniprot Accession Number P26902
Absorption
Active Site ACT_SITE 115; /note=Nucleophile
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.11.-
Enzyme Function FUNCTION: Hydrolyzes N-terminal residues in D-amino acid containing peptides. Among the tested substrates, the highest activities are with D-Ala-D-Ala and D-Ala-Gly-Gly. The physiological role is not clear.
Temperature Dependency
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 9-11.;
Pathway
nucleotide Binding
Features Active site (1); Beta strand (14); Chain (1); Helix (9); Metal binding (6); Sequence conflict (2); Turn (1)
Keywords 3D-structure;Aminopeptidase;Direct protein sequencing;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Sporulation;Zinc
Interact With
Induction INDUCTION: Nutrient deficiency conditions, which also induce sporulation.
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (1)
Cross Reference PDB 1HI9;
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 30,159
Kinetics
Metal Binding METAL 8; /note=Zinc 1; METAL 8; /note=Zinc 2; METAL 10; /note=Zinc 1; METAL 60; /note=Zinc 2; METAL 104; /note=Zinc 2; METAL 133; /note=Zinc 2
Rhea ID
Cross Reference Brenda 3.4.11.19;